CONSTRUCTION OF A BIOLUMINESCENT MYCOBACTERIUM AND ITS USE FOR ASSAY OF ANTIMYCOBACTERIAL AGENTS
CONSTRUCTION OF A BIOLUMINESCENT MYCOBACTERIUM AND ITS USE FOR ASSAY OF ANTIMYCOBACTERIAL AGENTS
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DOI：
10.1128/jcm.31.9.2251-2254.1993
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发表时间：
1993-09-01
影响因子：
9.4
通讯作者：
ROBERTS, IS
中科院分区：
文献类型：
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作者：
ANDREW, PW;ROBERTS, IS
为了显示，作为一个模型系统，分枝杆菌可以表达异源荧光素酶基因和生物发光可以是一种快速的方法来测量抗分枝杆菌活性，生物发光形式的耻垢分枝杆菌是通过转化与分枝杆菌-大肠杆菌穿梭载体含有来自哈氏弧菌的luxAB基因。可以使用生物发光M来真实的检测抗生素和杀菌剂的抗结核菌作用。恶臭
To show, as a model system, that mycobacteria can express heterologous luciferase genes and that bioluminescence can be a rapid method of measuring antimycobacterial activity, a bioluminescent form of Mycobacterium smegmatis was made by transformation with a Mycobacterium-Escherichia coli shuttle vector containing the luxAB genes from Vibrio harveyi. The antimycobacterial effects of antibiotics and biocides could be assayed in real time by using bioluminescent M. smegmatis.