SUPEROXIDE AND BONE-RESORPTION
SUPEROXIDE AND BONE-RESORPTION
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DOI：
10.1016/8756-3282(94)90821-4
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发表时间：
1994-07-01
期刊：
影响因子：
4.1
通讯作者：
RIES, WL
中科院分区：
文献类型：
--
作者：
KEY, LL;WOLF, WC;RIES, WL
超氧化物与氮蓝四唑的反应产生电子致密的二甲沉淀，其可用于定位超氧化物产生的区域。透射电子显微镜被用来证明，由氮蓝四唑与过量的超氧化物反应形成的二甲颗粒是电子致密的，而由过氧化氢产生的单甲颗粒没有。基于这些观察结果，通过证明破骨细胞膜和骨表面之间的电子致密的二甲颗粒，定位了沿破骨细胞-骨界面沿着形成的超氧化物。该反应产物的形成被超氧化物清除剂甲磺酸去铁胺-锰络合物（“绿色"络合物）抑制，证实了反应产物的特异性。清除剂也抑制骨吸收。在体外产生的高浓度的超氧化物在中性pH值降解成许多肽片段的骨钙素，表明超氧化物的能力，打破肽键。这些研究将超氧化物的产生定位于皱褶边缘空间，并表明破骨细胞-骨界面产生的超氧化物参与骨基质降解。
The reaction of superoxide with nitroblue tetrazolium produces an electron-dense diformazan precipitate which can be used to localize areas of superoxide production. Transmission electron microscopy was used to demonstrate that diformazan granules formed by the reaction of nitroblue tetrazolium with excess superoxide are electron dense, whereas monoformazan granules generated by hydrogen peroxide were not. On the basis of these observations, superoxide formed along the osteoclast-bone interface was localized by demonstrating the electron-dense diformazan granules between the osteoclastic membrane and the bone surface. The formation of this reaction product was inhibited by a superoxide scavenger, the deferoxamine mesylate-manganese complex (the ''green'' complex), confirming the specificity of the reaction product. The scavenger also inhibited bone resorption. High concentrations of superoxide generated in vitro at a neutral pH degraded osteocalcin into numerous peptide fragments, demonstrating the ability of superoxide to break peptide bonds. These studies localize superoxide production to the ruffled border space and suggest that superoxide generated at the osteoclast-bone interface is involved in bone matrix degradation.