Anti proliferative effect of adiponectin on MCF7 breast cancer cells: a potential hormonal link between obesity and cancer
Anti proliferative effect of adiponectin on MCF7 breast cancer cells: a potential hormonal link between obesity and cancer
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DOI：
10.1055/s-2007-956518
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发表时间：
2007-01-01
影响因子：
2.2
通讯作者：
Chrousos, G. P.
中科院分区：
文献类型：
--
作者：
Arditi, J. D.;Venihaki, M.;Chrousos, G. P.
脂联素是一种由脂肪组织分泌的激素，在人体血浆中以高浓度循环。矛盾的是，肥胖者的血浆脂联素水平大约比瘦人低50%。最近有报道称，低血浆脂联素水平与患乳腺癌和其他癌症的风险较高有关。肥胖和超重也与癌症死亡率的增加有关。为了验证脂联素对癌细胞发挥直接抗增殖和/或促凋亡作用的假设，我们使用了MCF7人乳腺腺癌细胞株。用四甲基偶氮唑盐比色法测定MCF7细胞的增殖率，用ELISA法测定DNA片段化程度来检测细胞的凋亡率。RT-PCR检测脂联素受体1(AdipoR1)和AdipoR2的mRNA表达。脂联素可降低MCF7细胞的增殖率，作用48-96小时后作用更为明显。受体表达的存在提示脂联素对细胞增殖的影响很可能是特异性的，并且是由脂联素受体介导的。脂联素在48小时内未诱导MCF7细胞凋亡。我们的结论是，脂联素抑制MCF7乳腺癌细胞的增殖，但不会导致细胞凋亡。这些数据表明，脂联素可能代表了肥胖和癌症之间的直接荷尔蒙联系。
Adiponectin, a hormone secreted by adipose tissue, circulates at high concentrations in human plasma. Paradoxically, plasma levels of adiponectin are approximately 50% lower in obese than in lean subjects. An association between low plasma levels of adiponectin and higher risk of developing breast and other cancers was recently reported. Obesity and overweight have also been associated with increased mortality from cancer. To test the hypothesis that adiponectin exerts direct anti proliferative and/or pro-apoptotic effects on cancer cells, we used the MCF7 human breast adenocarcinoma cell line. The proliferation rate of the MCF7 cells was measured using the MTT method, while apoptosis was examined by quantifying the DNA fragmentation using an ELISA assay. In addition, adiponectin receptor 1 (AdipoR1) and AdipoR2 mRNA expression was detected using RT-PCR. Adiponectin diminished the proliferation rate of MCF7 cells; this effect was significant after 48-96 hours of treatment. The presence of receptor expression suggested that the effect of adiponectin on cell proliferation was most likely specific and adiponectin receptor-mediated. Adiponectin induced no apoptosis of MCF7 cells over 48 hours. We conclude that adiponectin inhibits proliferation but causes no apoptosis of MCF7 breast cancer cells. These data suggest that adiponectin may represent a direct hormonal link between obesity and cancer.