Apoptosis during macrophage-dependent ocular tissue remodelling.
Apoptosis during macrophage-dependent ocular tissue remodelling.
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发表时间：
1994-12
期刊：
影响因子：
4.6
通讯作者：
Richard A. Lang;Marc Lustig;Fritz Francois;Meredith Sellinger;Heide Plesken
中科院分区：
文献类型：
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作者：
Richard A. Lang;Marc Lustig;Fritz Francois;Meredith Sellinger;Heide Plesken
我们已经描述了瞳孔膜退化过程中细胞死亡的性质和模式，瞳孔膜是在眼睛前房发现的一种发育短暂的毛细血管网络。该分析表明，瞳孔膜的细胞成分包括精细毛细血管复杂网络中的血管内皮细胞以及随之而来的巨噬细胞。毛细血管位于晶状体的前表面，由细胞外基质纤维组成的蛛网状网络保持相对位置，细胞外基质纤维随该结构的细胞成分一起退化。瞳孔膜消退过程中的细胞死亡是细胞凋亡的特征。具体来说，在血管内皮细胞中可以观察到含有浓缩染色质的凋亡小体，从瞳孔膜中分离的基因组DNA显示出凋亡细胞典型的核小体断裂模式。使用标记组织制剂中DNA片段的方法（TUNEL），我们评估了瞳孔膜消退过程中凋亡细胞死亡的总体模式。我们发现细胞凋亡既发生在健康血管的单个细胞中，也发生在整个毛细血管段同步发生。形态学分析和TUNEL分析均表明，毛细血管从一个结到另一个结，每次一个节段发生回缩。我们提出了一个模型来解释从这些实验和以前的实验中观察到的毛细血管退化模式（Lang和Bishop (1993) Cell 74, 453-462），巨噬细胞通过诱导细胞凋亡引起瞳孔膜退化过程中靶细胞的死亡。
We have characterized the nature and pattern of cell death during regression of the pupillary membrane, a developmentally transient capillary network found in the anterior chamber of the eye. This analysis has revealed that the cellular components of the pupillary membrane include vascular endothelial cells in an intricate network of fine capillaries as well as attendant macrophages. The capillaries are situated on the anterior surface of the lens and held in relative position by a cobweb-like meshwork of extracellular matrix fibres that regress along with the cellular components of this structure. Cell death during regression of the pupillary membrane is characteristic of apoptosis. Specifically, apoptotic bodies containing condensed chromatin can be observed in vascular endothelial cells and genomic DNA isolated from the pupillary membrane shows the nucleosomal fragmentation pattern typical of apoptotic cells. Using a method for labelling fragmented DNA in tissue preparations (TUNEL), we have assessed the overall pattern of apoptotic cell death during pupillary membrane regression. We find that apoptosis occurs either in single cells in healthy vessels or synchronously along the entire length of a capillary segment. Both morphological and TUNEL analysis indicate that capillary regression occurs from junction to junction one segment at a time. We propose a model to explain the pattern of capillary regression observed and conclude from these and previous experiments (Lang and Bishop (1993) Cell 74, 453-462), that during regression of the pupillary membrane, the macrophage elicits target cell death by inducing apoptosis.