Cell Culture on Low-Fluorescence and High-Resolution Photoresist
Cell Culture on Low-Fluorescence and High-Resolution Photoresist
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DOI：
10.3390/mi11060571
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发表时间：
2020-06-01
期刊：
影响因子：
3.4
通讯作者：
Suzuki, Takaaki
中科院分区：
文献类型：
--
作者：
Ueno, Hidetaka;Maruo, Katsuya;Suzuki, Takaaki
由光致抗蚀剂（一种聚合物）制成的2D和3D地形线索用于细胞培养和细胞分析。用于细胞分析的光致抗蚀剂提供适当细胞生长所需的表面条件，沿着具有宽范围和高精度的图案化性质，以及不影响荧光成像的低自发荧光。在本研究中，我们开发了一种具有上述特性的厚负性光刻胶SJI-001。我们评估了SJI-001的表面条件对细胞培养的影响。首先，我们研究了SJI-001的润湿性，其通过等离子体处理而改变，作为细胞接种前在塑料基底上进行的预处理。SJI-001比用于制造微机电系统（MEMS）的SU-8更化学稳定。此外，在未经处理的SJI-001上培养的贴壁HeLa细胞的倍增时间和粘附率分别为25.2小时和74%，从而表明其适合于SU-8的细胞培养。此外，我们使用SJI-001制造了具有3D晶格结构的细胞培养板，尺寸为3微米。接种在该板上的HeLa细胞在五天内保持附着。因此，SJI-001具有适合细胞培养的表面条件，并具有多种生物应用，包括用于细胞培养和细胞分析的微结构和细胞芯片。
2D and 3D topographic cues made of photoresist, a polymer, are used for cell culture and cell analysis. Photoresists used for cell analysis provide the surface conditions necessary for proper cell growth, along with patterning properties of a wide range and high precision, and low auto-fluorescence that does not affect fluorescence imaging. In this study, we developed a thick negative photoresist SJI-001 possessing the aforementioned properties. We evaluated the surface conditions of SJI-001 affecting cell culture. First, we studied the wettability of SJI-001, which was changed by plasma treatment, conducted as a pretreatment on a plastic substrate before cell seeding. SJI-001 was more chemically stable than SU-8 used for fabricating the micro-electromechanical systems (MEMS). Furthermore, the doubling time and adhesion rate of adherent HeLa cells cultured on untreated SJI-001 were 25.2 h and 74%, respectively, thus indicating its suitability for cell culture over SU-8. In addition, we fabricated a cell culture plate with a 3D lattice structure, three micrometers in size, using SJI-001. HeLa cells seeded on this plate remained attached over five days. Therefore, SJI-001 exhibits surface conditions suitable for cell culture and has several bioapplications including microstructures and cell chips for cell culture and cell analysis.