Prevalence of porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 in piglets after weaning on a commercial pig farm in Japan
Prevalence of porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 in piglets after weaning on a commercial pig farm in Japan
复制标题
DOI：
10.1111/j.1740-0929.2009.00706.x
复制
发表时间：
2010-01-01
影响因子：
2
通讯作者：
Ushida, Kazunari
中科院分区：
文献类型：
--
作者：
Sasaki, Kenichi;Tsukahara, Takamitsu;Ushida, Kazunari
为研究猪繁殖与呼吸综合征病毒（PRRSV）和猪圆环病毒2型（PCV2）在断奶当天及断奶后7、14、21、28、53、84、120 d血清中的浓度变化及抗体水平。采用实时荧光定量PCR和实时荧光定量PCR分别检测血清中PRRSV和PCV 2的浓度。用ELISA法检测猪繁殖与呼吸综合征病毒（PRRSV）和猪圆环病毒2型（PCV 2）的特异性抗体。在断奶后0天（dpw）未检测到PRRSV。随着血清中PRRSV浓度的增加，猪体内的特异性抗体开始增加，随后呈下降趋势。0 dpw时，28头猪中有12头检出PCV 2。PCV2浓度和特异性抗体的变化趋势与PRRSV相似。相关性分析表明，日增重的下降与PRRSV浓度的增加相一致。在0 dpw时，抗PRRSV或PCV 2抗体滴度较高的猪分别显示出较低的PRRSV或PCV 2水平。
To investigate the transition in concentration of porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2) and antibody for these viruses in serum, serum samples were collected from 29 pigs on weaning day and at 7, 14, 21, 28, 53, 84, and 120 days after weaning. The concentration of circulated PRRSV and PCV2 in serum was measured by real-time RT-PCR and real-time PCR, respectively. The specific antibody for PRRSV and PCV2 was measured using ELISA. PRRSV was not detected on 0 days post-weaning (dpw). The specific antibody for PRRSV began to increase as the concentration of PRRSV in serum increased, and the level of PRRSV then tended to decrease. PCV2 was detected in 12 of 28 pigs on 0 dpw. The concentration of PCV2 and the specific antibody for PCV2 showed a similar tendency to those of PRRSV. The correlation analysis suggests that a decline in the daily weight gain coincided with an increase in the PRRSV concentration. Pigs with a higher antibody titer against PRRSV or PCV2 on 0 dpw showed the lower level of PRRSV or PCV2, respectively.