Protection against bronchiolitis obliterans syndrome is associated with allograft CCR7+ CD45RA- T regulatory cells.
Protection against bronchiolitis obliterans syndrome is associated with allograft CCR7+ CD45RA- T regulatory cells.
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DOI：
10.1371/journal.pone.0011354
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发表时间：
2010-06-29
期刊：
影响因子：
3.7
通讯作者：
Belperio JA
中科院分区：
文献类型：
--
作者：
Gregson AL;Hoji A;Palchevskiy V;Hu S;Weigt SS;Liao E;Derhovanessian A;Saggar R;Song S;Elashoff R;Yang OO;Belperio JA
闭塞性细支气管炎综合征（BOS）是肺移植后长期生存的主要障碍，但目前缺乏早期检测和干预的标志物。鉴于调节性 T 细胞 (Treg) 在免疫调节中的作用，我们假设肺移植后支气管肺泡灌洗液 (BALF) 中 Treg 的频率可以预测 BOS 的后续发展。通过流式细胞术对取自 47 名肺移植受者的 70 个 BALF 样本进行 Treg 淋巴细胞亚群分析，同时对趋化因子进行 ELISA 测量。同种异体移植活检组织对感兴趣的趋化因子进行染色。 Treg 基本上都是 CD45RA−，并且总 Treg 频率与 BOS 结果不相关。大多数 Treg 为 CCR4+ 和 CD103−，这两个亚群均与 BOS 风险无关。相比之下，较高比例的 CCR7+ Treg 与 BOS 风险降低相关。此外，CCR7 配体 CCL21 与 CCR7+ Treg 频率相关，与 BOS 成反比。肺同种异体移植物中较高频率的 CCR7+ CD3+CD4+CD25hiFoxp3+CD45RA− 淋巴细胞与防止 BOS 的后续发展相关，表明该假定的 Treg 子集可能下调同种免疫。 CCL21 可能对于将这一独特亚群招募到同种异体肺移植物中至关重要，从而降低慢性排斥反应的风险。
Bronchiolitis obliterans syndrome (BOS) is the major obstacle to long-term survival after lung transplantation, yet markers for early detection and intervention are currently lacking. Given the role of regulatory T cells (Treg) in modulation of immunity, we hypothesized that frequencies of Treg in bronchoalveolar lavage fluid (BALF) after lung transplantation would predict subsequent development of BOS. Seventy BALF specimens obtained from 47 lung transplant recipients were analyzed for Treg lymphocyte subsets by flow cytometry, in parallel with ELISA measurements of chemokines. Allograft biopsy tissue was stained for chemokines of interest. Treg were essentially all CD45RA−, and total Treg frequency did not correlate to BOS outcome. The majority of Treg were CCR4+ and CD103− and neither of these subsets correlated to risk for BOS. In contrast, higher percentages of CCR7+ Treg correlated to reduced risk of BOS. Additionally, the CCR7 ligand CCL21 correlated with CCR7+ Treg frequency and inversely with BOS. Higher frequencies of CCR7+ CD3+CD4+CD25hiFoxp3+CD45RA− lymphocytes in lung allografts is associated with protection against subsequent development of BOS, suggesting that this subset of putative Treg may down-modulate alloimmunity. CCL21 may be pivotal for the recruitment of this distinct subset to the lung allograft and thereby decrease the risk for chronic rejection.
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介导同种异体移植耐受性的FOXP3+ T调节细胞的募集取决于CCR4趋化因子受体。
影响因子：
15.3
作者：
Lee, I;Wang, LQ;Wells, AD;Dorf, ME;Ozkaynak, E;Hancock, WW
通讯作者：
Hancock, WW
影响因子：
12.3
作者：
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通讯作者：
Zhang J
影响因子：
6.2
作者：
Bharat, Ankit;Narayanan, Kishore;Mohanakumar, Thalachallour
通讯作者：
Mohanakumar, Thalachallour
DOI：
10.1164/rccm.200409-1243oc
发表时间：
2005-06-01
影响因子：
24.7
作者：
Christie, JD;Kotloff, RM;Kimmel, SE
通讯作者：
Kimmel, SE
影响因子：
14.2
作者：
Allakhverdi, Zoulfia;Fitzpatrick, David;Delespesse, Guy
通讯作者：
Delespesse, Guy