SEX DIFFERENCES IN MYELIN-ASSOCIATED PROTEIN LEVELS WITHIN AND DENSITY OF PROJECTIONS BETWEEN THE ORBITAL FRONTAL CORTEX AND DORSAL STRIATUM OF ADULT RATS: IMPLICATIONS FOR INHIBITORY CONTROL
SEX DIFFERENCES IN MYELIN-ASSOCIATED PROTEIN LEVELS WITHIN AND DENSITY OF PROJECTIONS BETWEEN THE ORBITAL FRONTAL CORTEX AND DORSAL STRIATUM OF ADULT RATS: IMPLICATIONS FOR INHIBITORY CONTROL
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DOI：
10.1016/j.neuroscience.2015.05.029
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发表时间：
2015-08-06
期刊：
影响因子：
3.3
通讯作者：
Daniel, J. M.
中科院分区：
文献类型：
--
作者：
Bayless, D. W.;Daniel, J. M.
冲动的行为和决定往往会导致不良结果。损伤和神经成像研究表明，眼眶额叶皮质(OFC)和背侧纹状体(DSTR)在抑制控制中起关键作用。有人提出，更大的OFC输入到DSTR反映了自顶向下认知控制的增强和较少的冲动反应。我们之前曾报道过抑制控制方面的性别差异，即雌性大鼠比雄性大鼠犯更少的冲动错误。本研究的目的是调查年轻成年雄性和雌性大鼠的OFC和DSTR的差异。在实验1中，我们测量了OFC和DSTR中两种髓鞘相关蛋白的水平，即髓鞘碱性蛋白(MBP)和髓鞘蛋白脂蛋白(PLP)。Western印迹数据显示，与男性相比，女性在OFC中的MBP和PLP水平显著更高，而在DSTR中的水平相似。在实验2中，我们将顺行示踪剂生物素化葡聚糖胺(BDA)注入OFC，并测量DSTR中BDA的密度。用组织化学、光学显微镜成像和密度分析对BDA进行可视化。DSTR内的BDA密度女性明显高于男性，这表明从OFC到DSTR的投射女性可能比男性大。我们的结果提示了潜在的神经解剖学性别差异，这可能是已报道的雄性和雌性大鼠抑制控制水平差异的原因之一。(C)2015年IBRO。爱思唯尔有限公司出版。保留所有权利。
Impulsive actions and decisions often lead to undesirable outcomes. Lesion and neuroimaging studies have revealed that the orbital frontal cortex (OFC) and dorsal striatum (dSTR) play key roles in inhibitory control. It has been proposed that greater OFC input into the dSTR reflects enhanced top-down cognitive control and less impulsive responding. We previously reported a sex difference in inhibitory control, such that female rats make fewer impulsive errors than do male rats. The goal of the present study was to investigate differences in the OFC and dSTR of young adult male and female rats. In Experiment 1, we measured levels of two myelin-associated proteins, myelin basic protein (MBP) and myelin proteolipid protein (PLP), in the OFC and dSTR. Western blot data revealed that females had significantly higher levels of both MBP and PLP in the OFC but similar levels in the dSTR as compared to males. In Experiment 2, we infused the anterograde tracer, biotinylated dextran amine (BDA), into the OFC and measured the density of BDA in the dSTR. BDA was visualized using histochemistry followed by light microscopy imaging and densitometry analysis. Density of BDA in the dSTR was significantly greater in females as compared to males indicating that the projections from the OFC to dSTR may be greater in females as compared to males. Our results suggest a potential neuroanatomical sex difference that may contribute to the reported differences in inhibitory control levels of male and female rats. (C) 2015 IBRO. Published by Elsevier Ltd. All rights reserved.