Comparison of antibacterial and antifungal activities of 5-amino-2-mercaptobenzimidazole and functionalized NiO nanoparticles
Comparison of antibacterial and antifungal activities of 5-amino-2-mercaptobenzimidazole and functionalized NiO nanoparticles
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DOI：
10.1016/j.kijoms.2016.05.001
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发表时间：
2016
期刊：
影响因子：
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通讯作者：
S. Suresh;S. Karthikeyan;P. Saravanan;K. Jayamoorthy
中科院分区：
文献类型：
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作者：
S. Suresh;S. Karthikeyan;P. Saravanan;K. Jayamoorthy
研究了 5-氨基-2-巯基苯并咪唑 (AMB)、非功能化氧化镍 (n-NiO) 和功能化氧化镍 (f-NiO) 纳米粒子的抗菌和抗真菌活性，其中氧化镍纳米粒子的功能化是通过 AMB 进行的。合成的 n-NiO 和 f-NiO 纳米粒子的粒径经测量分别为 16.07 和 20.86 nm。 n-NiO和f-NiO纳米颗粒的XRD结果与JCPDS文件号89-5881中公布的NiO衍射图完全匹配，这表明功能化对晶体结构没有影响。 FT-IR 光谱研究表明，f-NiO 纳米粒子通过偶氮甲碱氮与 AMB 有效结合。此外，HR-SEM 和 EDAX 结果分别证实了表面形态和功能化。研究了分散在水中的 AMB、n-NiO 和 f-NiO 纳米颗粒的抗菌活性。采用琼脂充分扩散法评价其对金黄色葡萄球菌和铜绿假单胞菌的抗菌活性，并评价其对黑曲霉的抗真菌活性。与n-NiO纳米粒子和AMB相比，f-NiO纳米粒子表现出优异的抗菌和抗真菌活性。 f-NiO 纳米粒子的增强效果可能是由于 NiO 纳米粒子的分散性以及与细菌和真菌的膜和细胞内蛋白的相互作用增强。
5-Amino-2-mercaptobenzimidazole (AMB), non-functionalized nickel oxide (n-NiO), and functionalized nickel oxide (f-NiO) nanoparticles were studied for antibacterial and antifungal activities, where the functionalization of nickel oxide nanoparticles was carried out by AMB. The particle sizes of synthesized n-NiO and f-NiO nanoparticles were measured to be 16.07 and 20.86 nm, respectively. The XRD results of n-NiO and f-NiO nanoparticles were in perfect match with the diffraction pattern of NiO published in the JCPDS File No. 89-5881, which indicates that there is no effect on the crystal structure due to functionalization. FT-IR spectral studies show that f-NiO nanoparticles effectively bind with AMB by azomethine nitrogen. Furthermore, HR-SEM and EDAX results confirm the surface morphology and functionalization, respectively. The antimicrobial activity of AMB, n-NiO, and f-NiO nanoparticles dispersed in water was investigated. The antibacterial activity was evaluated against the bacteriaStaphylococcus aureusandPseudomonas aeruginosa, while the antifungal activity was evaluated against the fungiAspergillus nigerby using the agar well-diffusion method. The f-NiO nanoparticles exhibit excellent antibacterial and antifungal activities compared with n-NiO nanoparticles and AMB. The increased effect of f-NiO nanoparticles might be due to enhanced dispersibility and interaction of NiO nanoparticles with membrane and intracellular proteins of bacteria and fungi.