Isolation of a developmentally-regulated expressed sequence tag from bladder tissue using the mRNA differential display.
Isolation of a developmentally-regulated expressed sequence tag from bladder tissue using the mRNA differential display.
复制标题
使用 mRNA 差异显示从膀胱组织中分离发育调节的表达序列标签。
DOI：
10.1080/15216549600201643
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Macarak,EJ
中科院分区：
文献类型：
--
作者：
Chaqour,B;Howard,PS;Macarak,EJ
In order to gain insight into the molecular and cellular events that govern the structural and the functional properties in developing organs, we have conducted a study to identify genes that have a temporally‐restricted expression in the bladder wall during fetal development. We utilized the mRNA differential display technique and compared the pattern of gene expression during the first, the second and the third trimester of gestation. We cloned and sequenced a cDNA fragment (bld‐10) which was expressed during the second and third trimester but consistently absent during the first trimester. The bld‐10 sequence is not related to any known gene in the GenBank database but has significant homology (89%) with human expressed sequence tag (EST) that has been cloned from human fetal heart and brain libraries. When used in Northern‐blot hybridization as a probe, the fragment bld‐10 generates two hybridization signals of 3.1 and 4.0 kb, that are minimally expressed during the first trimester of gestation and up‐regulated in the second and third trimester. Differential expression of this gene may be responsible for some of the profound changes which occur during organ development.
