DEFORMABILITY OF STORED RED-BLOOD-CELLS - RELATIONSHIP TO DEGREE OF PACKING
DEFORMABILITY OF STORED RED-BLOOD-CELLS - RELATIONSHIP TO DEGREE OF PACKING
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DOI：
10.1046/j.1537-2995.1982.22282177134.x
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发表时间：
1982-01-01
期刊：
影响因子：
2.9
通讯作者：
SHOHET, SB
中科院分区：
文献类型：
--
作者：
CARD, RT;MOHANDAS, N;SHOHET, SB
在柠檬酸盐-磷酸盐-葡萄糖防腐剂中，在不同程度的细胞包装下，血液储存42天期间，使用Ektacytometer测量[人]红细胞变形性。观察到的变形能力的变化进行了研究，在储存过程中的红细胞ATP水平，脂质含量，渗透脆性和红细胞压积的关系。在等渗培养基中测量的全细胞变形能力的降低直到细胞ATP水平降低至初始值的< 30%才发生。由于基质消耗更快，储存期间变形性损失的速率随着细胞堆积程度的增加而增加。当在低渗介质中进行变形性测量时，等渗变形性的损失进一步放大，表明表面积与体积比的减少是储存红细胞变形性降低的主要原因。显著的球形红细胞增多、膜脂含量降低和渗透脆性增加证实了在压积红细胞中观察到的损失，这表明可能需要仔细控制储存红细胞压积，以避免细胞变形性和可能的输血后活力的损失。
An ektacytometer eas used to measure [human] red blood cell deformability during blood storage for 42 days at different degrees of cell packing in citrate-phosphate-dextrose preservative. Observed changes in deformability were studied in relationship to red blood cell ATP levels, lipid content, osmotic fragility and hematocrit during storage. Decrease in whole cell deformability as measured in isotonic medium did not occur until cellular ATP levels were reduced to < 30% of initial values. The rate of deformability loss during storage increased with increased degrees of cell packing because of more rapid substrate depletion. The loss in isotonic deformability was further magnified when deformability measurements were carried out in hypotonic media, suggesting that a reduction in surface area-to-volume ratio was the dominant cause of the reduced deformability of stored red blood cells. Marked spherocytosis, decreased membrane lipid content and increased osmotic fragility confirmed loss observed in packed red blood cell suggests that careful control of storage hematocrit may be necessary to avoid loss of cellular deformability and possibly posttransfusion viability.