Presence of Epstein-Barr virus in extranodal T-cell lymphomas: differences in relation to site.
Presence of Epstein-Barr virus in extranodal T-cell lymphomas: differences in relation to site.
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结外 T 细胞淋巴瘤中 Epstein-Barr 病毒的存在：与部位相关的差异。
DOI：
10.1182/blood.v83.6.1612.1612
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发表时间：
1994
期刊：
影响因子：
20.3
通讯作者：
R. Willemze
中科院分区：
文献类型：
--
作者：
P. D. de Bruin;M. Jiwa;J. Oudejans;P. Van Der Valk;P. van Heerde;J. Sabourin;G. Csanaky;P. Gaulard;A. Noorduyn;R. Willemze
形态相似但起源部位不同的T细胞淋巴瘤具有不同的临床表现。对这一发现的理论解释源于这样的假设，即非霍奇金淋巴瘤（NHL）是涉及组织限制性淋巴细胞的免疫反应的肿瘤等价物。这一假说还意味着，来自不同位点的T-NHL在其发生上不同，因此可能在致癌基因表达、粘附分子表达或某些DNA/RNA病毒序列的存在上不同。因此，我们研究了起源于不同部位（即鼻）的具有相似形态的T细胞淋巴瘤，（n = 5;所有多形性小细胞或中细胞和大细胞T细胞淋巴瘤[PTL]），皮肤（PTL，n = 6;间变性大细胞[ALCL]，n = 11），肠道（PTL，n = 8; ALCL，n = 4）和肺（PTL，n = 6），在DNA、RNA（EBER 1和EBER 2）和蛋白质水平（LMP-1）的EB病毒（EBV）的存在。采用双染色技术检测EBER 1/2、LMP-1和分化标志物在单细胞水平的表达。在5例鼻T细胞淋巴瘤、17例原发性皮肤T细胞淋巴瘤、1例胃肠道T细胞淋巴瘤（ALCL）和2例肺T细胞淋巴瘤中发现了大量EBER 12阳性肿瘤细胞（> 100个/中倍视野[mpf]）。因此，这些淋巴瘤被称为EBV相关淋巴瘤。与我们早期在淋巴结中的发现相反，没有发现淋巴结淋巴瘤，只有少数EBV阳性肿瘤细胞。聚合酶链反应（PCR）检测5例EBV阳性的胃肠道病例，EBER 1/2仅见于散发性非肿瘤性反应性淋巴细胞。18例PTL和1例ALCL存在血管中心性，但与EBV的存在无关。这些结果表明，EB病毒在淋巴结T细胞淋巴瘤的存在是站点限制性的，并主张具有相似形态但起源于不同站点的T细胞淋巴瘤的不同发病机制。在这些EBV相关淋巴瘤中，大多数肿瘤细胞中存在EBV，这表明EBV在这些淋巴瘤的发病机制中可能是重要的。
T-cell lymphomas with similar morphology but with different sites of origin have a different clinical behavior. The theoretical explanation for this finding originates from the hypothesis that non-Hodgkin's lymphomas (NHLs) are neoplastic equivalents of immunological reactions involving tissue-restricted lymphocytes. This hypothesis also implies that T-NHLs originating from different sites differ in their genesis, and thus may differ in oncogen expression, expression of adhesion molecules, or presence of certain DNA/RNA viral sequences. Therefore, we have investigated in T-cell lymphomas with similar morphology originating from different sites, ie, nose (n = 5; all pleomorphic small- or medium- and large-cell T-cell lymphomas [PTL]), skin (PTL, n = 6; anaplastic large-cell [ALCL], n = 11), gut (PTL, n = 8; ALCL, n = 4), and lung (PTL, n = 6), the presence of Epstein-Barr virus (EBV) at the DNA, RNA (EBER 1 and EBER 2), and protein level (LMP-1). A double-staining technique was used to detect EBER 1/2, LMP-1, and differentiation markers at the single-cell level. High numbers of EBER 1/2-positive tumor cells (> 100 per medium power field [mpf]) were found in five of five nasal T-cell lymphomas, none of 17 primary cutaneous T-cell lymphomas, one of 12 gastrointestinal T-cell lymphomas (ALCL), and two of six pulmonary T-cell lymphomas. These lymphomas are therefore called EBV-associated lymphomas. In contrast to our earlier findings in lymph nodes, no extranodal lymphomas were found, with only a few EBV-positive tumor cells. Five gastrointestinal cases positive for EBV by polymerase chain reaction (PCR) showed that EBER 1/2 was only found in sporadic nonneoplastic, ie, reactive lymphocytes. Angiocentricity was present in 18 PTL and one ALCL, but not associated with the presence of EBV. These results indicate that the presence of EBV in extranodal T-cell lymphomas is site-restricted and argues for a different pathogenesis of T-cell lymphomas with similar morphology but originating from different sites. The presence of EBV in most tumor cells in these EBV-associated lymphomas suggests that when present, EBV might be important in the pathogenesis of these lymphomas.
DOI：
10.1073/pnas.87.6.2244
发表时间：
1990-03-01
影响因子：
11.1
作者：
KISHIMOTO, TK;JUTILA, MA;BUTCHER, EC
通讯作者：
BUTCHER, EC
DOI：
--
发表时间：
1990
期刊：
The American journal of pathology
影响因子：
--
作者：
L. Picker;S. Michie;L. Rott;E. Butcher
通讯作者：
L. Picker;S. Michie;L. Rott;E. Butcher
爱泼斯坦-巴尔病毒和霍奇金病。
DOI：
--
发表时间：
1991
期刊：
The American journal of pathology
影响因子：
--
作者：
Weiss,LM;Chen,YY;Liu,XF;Shibata,D
通讯作者：
Shibata,D