Characteristics of galactose transport in Saccharomyces cerevisiae cells and reconstituted lipid vesicles
Characteristics of galactose transport in Saccharomyces cerevisiae cells and reconstituted lipid vesicles
复制标题
酿酒酵母细胞和重组脂质囊泡中半乳糖转运的特征
DOI：
--
复制
发表时间：
1989
影响因子：
3.2
通讯作者：
V. P. Cirillo
中科院分区：
文献类型：
--
作者：
J. Ramos;K. Szkutnicka;V. P. Cirillo
半乳糖上的生长诱导两个运输过程，高亲和力和低亲和力过程。两个过程的比较的最重要的结果是：（i）两者都依赖于GAL 2表达，（ii）仅高亲和力过程需要半乳糖激酶，（iii）两者都被分解代谢物失活下调，（iv）两者都不被羰腈-对-三氟甲氧基-苯-腙显著抑制，（v）两者都不被银或氯化汞差异抑制，和（vi）在融合的磷脂膜囊泡中重建具有与全细胞的低亲和力过程的Km更接近的Km的转运活性。
Growth on galactose induces two transport processes, a high-affinity and a low-affinity process. The most important results of a comparison of the two processes were that (i) both depended on GAL2 expression, (ii) only the high-affinity process required galactokinase, (iii) both were down-regulated by catabolite inactivation, (iv) neither was significantly inhibited by carbonyl cyanide-p-trifluoromethoxy-phenyl-hydrazone, (v) neither was differentially inhibited by silver nitrate or mercuric chloride, and (vi) transport activity with a Km closer to that of the low-affinity process of whole cells was reconstituted in fused phospholipid membrane vesicles.