Synthesis, characterization, photophysical properties, and biological labeling studies of a series of luminescent rhenium(I) polypyridine maleimide complexes
Synthesis, characterization, photophysical properties, and biological labeling studies of a series of luminescent rhenium(I) polypyridine maleimide complexes
复制标题
DOI：
10.1021/ic010602z
复制
发表时间：
2002-01-14
影响因子：
4.6
通讯作者：
Cheung, KK
中科院分区：
文献类型：
--
作者：
Lo, KKW;Hui, WK;Cheung, KK
本文报道了一系列双（1）多吡啶马来酰亚胺配合物[Re（N-N）（CO）（3）]的合成、表征和物理性质（py-3-mal）]（CF 3SO 3）[N-N = 1，10-菲咯啉，phen（1），2，9-二甲基-1，10-菲咯啉，2，9-Me-2-phen（2），3，4，7，8-四甲基-1，10-菲咯啉，3，4，7，8-Me-4-phen（3），4，7-diphenyl-1，10-phen（4），2，9-dimethyl-4，7-diphenyl-1，10-phen（5），2，2'-biquinandrile，biq（6）; py-3-mal = N-（3-pyridyl）maleimide]。研究了配合物2的X-射线晶体结构。在298 K下，激发后，配合物在流体溶液中表现出强烈和长寿命的光致发光。发射波长范围从514到654 nm，并且发射寿命落在微秒时间尺度内。发光被指定为源自金属-配体电荷转移MLCT [dpi（Re）-pi*（二亚胺）]三重激发态。由于马来酰亚胺基团可以与巯基反应以形成稳定的硫醚部分，这些络合物已经用作硫醇化寡核苷酸、谷胱甘肽以及牛血清白蛋白和人血清白蛋白的硫醇特异性发光标记物。标记的生物物种的光致发光特性也进行了研究。
We report the synthesis, characterization, and photophysical properties of a series of rhenium(l) polypyridine maleimide complexes [Re(N-N)(CO)(3)(py-3-mal)](CF3SO3) [N-N = 1,10-phenanthroline, phen (1), 2,9-dimethyl-1,10-phenanthroline, 2,9-Me-2-phen (2), 3,4,7,8-tetramethyl-1,10-phenanthroline, 3,4,7,8-Me-4-phen (3), 4,7-diphenyl-1,10-phenanthroline, 4,7-Ph-2-phen (4), 2,9-dimethyl-4,7-diphenyl-1,10-phenanthroline, 2,9-Me-2-4,7-Ph-2-phen (5), 2,2'-biquinonine, biq (6); py-3-mal = N-(3-pyridyl)maleimide]. The X-ray crystal structure of complex 2 has been investigated. Upon excitation, the complexes exhibit intense and long-lived photoluminescence in fluid solutions at 298 K. The emission wavelengths range from 514 to 654 nm, and the emission lifetimes fall in the microsecond time scale. The luminescence is assigned to originate from a metal-to-ligand charge-transfer MLCT [dpi(Re) --> pi*(diimine)] triplet excited state, As the maleimide group can react with the sulfhydryl group to form a stable thioether moiety, these complexes have been used as thiol-specific luminescent labels for a thiolated oligonucleotide, glutathione, and bovine serum albumin and human serum albumin, The photoluminescence properties of the labeled biological species have also been investigated.