Comparative virulence of urinary and bloodstream isolates of extra-intestinal pathogenic Escherichia coli in a Galleria mellonella model
Comparative virulence of urinary and bloodstream isolates of extra-intestinal pathogenic Escherichia coli in a Galleria mellonella model
复制标题
DOI：
10.4161/21505594.2014.988095
复制
发表时间：
2015-02-01
期刊：
影响因子：
5.2
通讯作者：
Wareham, David W.
中科院分区：
文献类型：
--
作者：
Ciesielczuk, Holly;Betts, Jonathon;Wareham, David W.
肠外致病性大肠杆菌(ExPEC)是世界范围内尿路感染和菌血症的重要原因。目前，还没有单一的毒力因子或ExPEC谱系被确定为严重的肠外感染和/或尿毒症的唯一因素。梅氏Galeria mellonella最近被建立为研究ExPEC相对毒力的简单模型。在这项研究中，我们通过测量死亡率(幼虫存活率)、免疫识别/反应(黑色素产生)和细胞损伤(乳酸脱氢酶产生)来研究40株特征良好的ExPEC菌株在梅隆革兰氏菌中的毒力。虽然尿路和血液分离株的死亡率相似，但社区相关感染、复杂性尿路感染和尿源性菌血症的死亡率较高。ST131分离株和那些具有AFA/drA、ompT和O6血清组的分离株也与毒力增强有关。
Extra-intestinal pathogenic Escherichia coli (ExPEC) are a significant cause of urinary tract infections and bacteraemia worldwide. Currently no single virulence factor or ExPEC lineage has been identified as the sole contributor to severe extra-intestinal infection and/or urosepsis. Galleria mellonella has recently been established as a simple model for studying the comparative virulence of ExPEC. In this study we investigated the virulence of 40 well-characterized ExPEC strains, in G. mellonella, by measuring mortality (larvae survival), immune recognition/response (melanin production) and cell damage (lactate dehydrogenase production). Although mortality was similar between urinary and bloodstream isolates, it was heightened for community-associated infections, complicated UTIs and urinary-source bacteraemia. Isolates of ST131 and those possessing afa/dra, ompT and serogroup O6 were also associated with heightened virulence.