Systems analysis of membrane transport and homeostasis in stomatal guard cells
Systems analysis of membrane transport and homeostasis in stomatal guard cells
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气孔保卫细胞膜运输和稳态的系统分析
DOI：
10.1016/j.cbpa.2009.04.416
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Chen Z
中科院分区：
文献类型：
--
作者：
Chen Z
保卫细胞在调节植物光合CO2吸收和蒸腾失水中起着重要作用。气孔运动的驱动机制已经在表皮、完整叶片、原生质体和膜斑的保卫细胞水平上得到了深入的研究。然而，我们的知识的内部机制，控制动态连续的气孔开度是非常贫穷的。利用系统分析，我们正在弥合这一差距，了解气孔孔径的动态范围和质膜和液泡膜运输的调节。对我们来说，一个重要的焦点是了解如何运输整合与动态平衡在应对环境压力，以及如何这样的整合确定的“设定点”气孔开度。为此，正在开发一个软件平台，用于对涉及离子和溶质通道、泵、载体和协同转运蛋白的保卫细胞膜转运和体内平衡进行定量数学建模。基于保卫细胞转运蛋白的详细动力学信息，正在用C++语言对关键转运蛋白的各个模型进行编程。同时，我们正在使用成像，电生理学和相关技术来检查保卫细胞的振荡行为。这些研究将用于测试和验证我们模型的预测。
Guard cells play a vital role in regulating photosynthetic CO2 uptake and transpirational water loss from plants. The mechanisms that drive stomatal movement have been intensively studied at the level of the guard cells in epidermal peels and in intact leaves, protoplasts and membrane patches. However, our knowledge of the internal mechanisms that control the dynamic continuum of stomatal apertures is very poor. Using systems analysis, we are bridging this gap in understanding of the dynamic range of stomatal apertures and its regulation by plasma membrane and tonoplast transport. An important focus for us is to understand how transport integrates with homeostasis in response to environmental stress and how such integration determines the'set point'for stomatal aperture. To this end, a software platform is under development for quantitative mathematical modelling of guard cell membrane transport and homeostasis involving ion and solute channels, pumps, carriers and co-transporters. Individual models of the key transporters are being programmed in the C++ language, based on the detailed kinetic information of guard cell transporters. Concurrently, we are using imaging, electrophysiology and related techniques to examine the oscillatory behaviour of guard cells. These studies will be used to test and validate our model's predictions.