The ydaO motif is an ATP-sensing riboswitch in Bacillus subtilis
The ydaO motif is an ATP-sensing riboswitch in Bacillus subtilis
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DOI：
10.1038/nchembio.1095
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发表时间：
2012-12-01
影响因子：
14.8
通讯作者：
Fedor, Martha J.
中科院分区：
文献类型：
--
作者：
Watson, Peter Y.;Fedor, Martha J.
我们报告什么是我们所知的第一个天然RNA，调节基因表达的细胞内ATP。使用生化筛选，我们发现，几个假定的核糖开关结合ATP在体外。在枯草芽孢杆菌中，ydaO基序特异性结合ATP并响应ATP浓度调节内源基因和报告基因的表达。这一发现证明了RNA在调节基因表达以响应细菌中的能量平衡中的作用。
We report what is to our knowledge the first natural RNA that regulates gene expression in response to intracellular ATP. Using a biochemical screen, we found that several putative riboswitches bind ATP in vitro. The ydaO motif specifically bound ATP and regulated expression of endogenous and reporter genes in response to ATP concentrations in Bacillus subtilis. This discovery demonstrates a role for RNAs in regulating gene expression in response to energy balance in bacteria.