On the use of differential solubility in aqueous ethanol solutions to narrow the DP range of food-grade starch hydrolysis products
On the use of differential solubility in aqueous ethanol solutions to narrow the DP range of food-grade starch hydrolysis products
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DOI：
10.1016/j.foodchem.2015.10.120
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发表时间：
2016-04-15
期刊：
影响因子：
8.8
通讯作者：
Penner, Michael H.
中科院分区：
文献类型：
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作者：
Balto, Amy S.;Lapis, Trina J.;Penner, Michael H.
大量研究集中在了解由葡萄糖、麦芽糖、麦芽低聚糖 (MOS) 和麦芽多糖 (MPS) 组成的淀粉水解产物 (SHP) 的功能。这项研究中的一个混杂因素是市售小水电的高分子分散性。本文提出的研究描述了用于降低此类产品的分散性的灵活分馏方法。这是通过根据其成分糖在乙醇水溶液中的不同溶解度对玉米糖浆固体（CSS）制剂进行分级来实现的。从选定的分级分离中获得的产物的特征在于聚合度（DP；液相色谱）、葡萄糖当量（还原糖测定）和支化率（NMR）。使用高 (P90%) 乙醇提取剂优先从 CSS 中去除葡萄糖和麦芽糖。通过重复 70% 乙醇提取，获得了 MOS 范围相对较窄、DP MPS 较低和 DP MPS 较高的制剂。与支链相反，在所有测试条件下，优先提取线性 MOS 和 MPS。 (C) 2015 Elsevier Ltd. 保留所有权利。
Considerable research is focused on understanding the functionality of starch hydrolysis products (SHP) consisting of glucose, maltose, maltooligosaccharides (MOS), and maltopolysaccharides (MPS). A confounding factor in this research is the high molecular dispersity of commercially available SHP. The study presented herein characterizes a flexible fractionation approach for lowering the dispersity of such products. This was accomplished by fractionating a corn syrup solids (CSS) preparation based on the differential solubility of its component saccharides in aqueous-ethanol solutions. Products obtained from selected fractionations were characterized with respect to degree of polymerization (DP; liquid chromatography), dextrose equivalency (reducing sugar assays), and prevalence of branching (NMR). Glucose and maltose were preferentially removed from CSS using high (P90%) ethanol extractants. Preparations with relatively narrow ranges of MOS, lower DP MPS, and higher DP MPS were obtained through repetitive 70%-ethanol extractions. Linear, as opposed to branched, MOS and MPS were preferentially extracted under all conditions tested. (C) 2015 Elsevier Ltd. All rights reserved.