Single capture bright field and off-axis digital holographic microscopy.
Single capture bright field and off-axis digital holographic microscopy.
复制标题
单次捕获明场和离轴数字全息显微镜。
DOI：
10.1364/ol.478674
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发表时间：
2022
期刊：
影响因子：
3.6
通讯作者：
B. Kemper
中科院分区：
文献类型：
--
作者：
J. Picazo;Á. Barroso;S. Ketelhut;J. Schnekenburger;V. Micó;B. Kemper
我们报告了一个单一的捕获方法，同时非相干明场（BF）和激光为基础的定量相位成像（QPI）。共光路数字全息显微术（DHM）与商业光学显微镜的光路内的BF成像并行地实现，以实现白色光图像和数字离轴全息图的空间复用记录，其随后被数字解复用。所提出的多峰概念的性能首先通过对微球的调查来确定。然后，应用无标记的双模式QPI和BF成像的活胰腺肿瘤细胞的证明。
We report on a single capture approach for simultaneous incoherent bright field (BF) and laser-based quantitative phase imaging (QPI). Common-path digital holographic microscopy (DHM) is implemented in parallel with BF imaging within the optical path of a commercial optical microscope to achieve spatially multiplexed recording of white light images and digital off-axis holograms, which are subsequently numerically demultiplexed. The performance of the proposed multimodal concept is firstly determined by investigations on microspheres. Then, the application for label-free dual-mode QPI and BF imaging of living pancreatic tumor cells is demonstrated.