Rapid, Label-Free, Electrical Whole Blood Bioassay Based on Nanobiosensor Systems
Rapid, Label-Free, Electrical Whole Blood Bioassay Based on Nanobiosensor Systems
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DOI：
10.1021/nn2035796
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发表时间：
2011-12-01
期刊：
影响因子：
17.1
通讯作者：
Zhou, Chongwu
中科院分区：
文献类型：
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作者：
Chang, Hsiao-Kang;Ishikawa, Fumiaki N.;Zhou, Chongwu
近年来，基于纳米线生物传感器的生物标志物检测吸引了大量的研究努力。然而，只有非常有限的研究工作已经直接从生理流体中检测生物标志物，主要是因为由介质的复杂性引起的挑战。这种限制显著降低了上述纳米生物传感器产生的实际影响。在这项研究中，我们展示了一种基于In2O3纳米线的生物传感系统，该系统能够直接从手指针刺收集的人类全血中快速，无标记，电检测癌症生物标志物。当在全血中进行主动测量时，钝化纳米线表面成功地阻断了非特异性结合诱导的信号。与裸器械相比，钝化器械显示由血清中蛋白质和其他生物材料的非特异性结合诱导的信号明显更小，对靶生物标志物的灵敏度更高。钝化传感器对全血中生物标志物的检测限与相同离子强度下纯化缓冲溶液中相同分析物的检测限相似，这表明复杂介质中设备性能的下降幅度极小。然后，我们证明了使用该传感系统在临床上有意义的浓度下以高可靠性从通过手指采集的全血中检测多种癌症生物标志物。
Biomarker detection based on nanowire biosensors has attracted a significant amount of research effort in recent years. However, only very limited research work has been directed toward biomarker detection directly from physiological fluids mainly because of challenges caused by the complexity of media. This limitation significantly reduces the practical impact generated by the aforementioned nanobiosensors. In this study, we demonstrate an In2O3 nanowire-based biosensing system that is capable of performing rapid, label-free, electrical detection of cancer biomarkers directly from human whole blood collected by a finger prick. Passivating the nanowire surface successfully blocked the signal induced by nonspecific binding when performing active measurement in whole blood. Passivated devices showed markedly smaller signals induced by nonspecific binding of proteins and other biomaterials in serum and higher sensitivity to target biomarkers than bare devices. The detection limit of passivated sensors for biomarkers in whole blood was similar to the detection limit for the same analyte in purified buffer solutions at the same ionic strength, suggesting minimal decrease In device performance in the complex media. We then demonstrated detection of multiple cancer biomarkers with high reliability at clinically meaningful concentrations from whole blood collected by a finger pick using this sensing system.