Evaluation of various antigenic substrates for the detection of antinuclear antibodies.
Evaluation of various antigenic substrates for the detection of antinuclear antibodies.
复制标题
评估用于检测抗核抗体的各种抗原底物。
DOI：
10.1159/000250192
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
A. Varelzidis
中科院分区：
文献类型：
--
作者：
A. Tosca;J. Hatzis;A. Haidas;A. Varelzidis
本文对间接免疫荧光法检测核抗体的几种底物进行了评价。我们检查了白色雄性小鼠肝印片，白色雌性妊娠小鼠肝印片，由雄性小鼠肝、肾和胃壁切片组成的复合基质，银屑病患者的皮肤切片，鳞状细胞癌的皮肤切片，雄性兔脾印片，健康成年人的皮肤切片，基底细胞上皮瘤的皮肤切片，扁平苔藓患者的皮肤切片，雄性小鼠脾印和雄性豚鼠脾印。为了评估底物，我们使用了4种来自胶原病患者的选定血清。观察到某些类型的抗核抗体对某些底物表现出更大的亲和力，并且荧光核模式可能在连续稀释时发生变化。在研究的所有底物中，认为白色雄性小鼠肝脏印迹和雄性家兔脾脏印迹对于常规目的最有效，因为它们既敏感又容易获得。
The present study deals with the evaluation of some substrates for the detection of nuclear antibodies by the indirect immunofluorescence method. We have examined white male mouse liver imprint, white female pregnant mouse liver imprint, compound substrate consisting of male mouse liver, kidney and gastric wall sections, skin sections from psoriatic patients, skin sections from squamous cell carcinomata, male rabbit spleen imprint, skin sections from healthy adults people, skin sections from basal cell epitheliomata, skin sections from patients with lichen planus, male mouse spleen imprint and male guinea pig spleen imprint. To evaluate the substrates, we have employed 4 selected sera from patients with collagen disease. It was observed that some types of antinuclear antibodies demonstrate greater affinity for certain substrates and the fluorescent nuclear pattern might change on serial dilutions. From all the substrates studied, the white male mouse liver imprint and the male rabbit spleen imprint were considered as the most efficient for routine purpose as they are both sensitive and easily obtainable.