Syntrophic interactions drive the hydrogen production from glucose at low temperature in microbial electrolysis cells
Syntrophic interactions drive the hydrogen production from glucose at low temperature in microbial electrolysis cells
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共养相互作用驱动微生物电解池中低温葡萄糖产氢
DOI：
10.1016/j.biortech.2012.08.040
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发表时间：
2012-11-01
影响因子：
11.4
通讯作者：
Logan, Bruce E.
中科院分区：
文献类型：
--
作者：
Lu, Lu;Xing, Defeng;Logan, Bruce E.
H-2可以通过在中温温度下发酵从葡萄糖中获得，但在这里，我们证明了氢也可以在低温下使用微生物电解池（MEC）从葡萄糖中获得。在单室MEC中，在4摄氏度下由葡萄糖产生H-2，产率约为6 mol H-2 mol（-1）葡萄糖，速率为0.25 +/- 0.03-0.37 +/- 0.04 m（3）H-2 m（-3）d（-1）。16 S rRNA基因焦磷酸测序和电化学分析表明，葡萄糖发酵与胞外产电菌氧化发酵产物相结合的互养相互作用是低温下电流产生的主要途径，而不是胞外产电菌直接氧化葡萄糖的途径。在25 ℃反应器中发现的另一种互养相互作用，甲烷生成和同型乙酸生成，在4 ℃的MEC中没有检测到。这些结果证明了在低温下在MEC中从丰富的碳水化合物生物质生产H-2的可行性。(C)2012爱思唯尔有限公司保留所有权利。
H-2 can be obtained from glucose by fermentation at mesophilic temperatures, but here we demonstrate that hydrogen can also be obtained from glucose at low temperatures using microbial electrolysis cells (MECs). H-2 was produced from glucose at 4 degrees C in single-chamber MECs at a yield of about 6 mol H-2 mol(-1) glucose, and at rates of 0.25 +/- 0.03-0.37 +/- 0.04 m(3) H-2 m(-3) d(-1). Pyrosequencing of 16S rRNA gene and electrochemical analyses showed that syntrophic interactions combining glucose fermentation with the oxidization of fermentation products by exoelectrogens was the predominant pathway for current production at a low temperature other than direct glucose oxidization by exoelectrogens. Another syntrophic interaction, methanogenesis and homoacetogenesis, which have been found in 25 degrees C reactors, were not detected in MECs at 4 degrees C. These results demonstrate the feasibility of H-2 production from abundant biomass of carbohydrates at low temperature in MECs. (C) 2012 Elsevier Ltd. All rights reserved.