Multi-omic studies on the toxicity variations in effluents from different units of reclaimed water treatment.
Multi-omic studies on the toxicity variations in effluents from different units of reclaimed water treatment.
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DOI：
10.1016/j.watres.2021.117874
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发表时间：
2021-11
期刊：
影响因子：
12.8
通讯作者：
Yongfeng Deng;Yan Zhang;H. Ren
中科院分区：
文献类型：
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作者：
Yongfeng Deng;Yan Zhang;H. Ren
传统的中水处理技术不能有效去除微污染物。虽然这些残留微污染物的健康风险已经通过模型计算进行了评估，但很少进行基于动物的研究，这对于风险验证是必不可少的。采用转录物、代谢组学等生化技术，对南京市某再生水厂氧化沟(OD)、混凝池(CT)、曝气生物滤池(BAF)和紫外线消毒池(UV)出水的毒性变化进行了研究。在处理单元沿线没有观察到明显的毒性降低趋势。与对照组和其他三个处理单元的出水相比，长期暴露在再生水(UV出水)中会加剧小鼠的氧化应激，并导致脂代谢和免疫反应异常。此外，多组生物标志物(4个差异表达基因和8个显著变化的代谢物)与残留微污染物(40种半挥发性有机化合物和6N-亚硝胺)之间存在显著的相关性。我们的结果强烈表明，再生水的健康风险主要是由残留的微污染物引起的，需要更先进的处理和更严格的排放标准来确保再生水的安全。
The conventional reclaimed water technologies could not effectively remove the micropollutants. Although the health risks of these residual micropollutants have been evaluated by model calculation, few animal-based studies have been performed, which is essential for risk verification. Here, we used transcriptomic, metabolomic, and other biochemical techniques to reveal the toxicity variations of effluents from oxidation ditch (OD), coagulation tank (CT), biological aerated filter (BAF), and ultraviolet disinfection pool (UV) in a reclaimed water plant located in Nanjing, China. No evident toxicity reduction trend was observed along the treatment units. Compared with control and other three treatment unit effluents, long-term exposure to reclaimed water (UV effluent) aggravated oxidative stress in mice and induced abnormal lipid metabolism and immune response. Moreover, striking correlations were identified between multi-omic biomarkers (4 differentially expressed genes and 8 significant changes metabolites) and residual micropollutants (40 semi-volatile organic compounds and 6N-nitrosamines). Our results strongly highlight that the health risks of reclaimed water are mainly induced by the residual micropollutants, and more advanced treatments and stringent discharge standards are needed to ensure the safety of reclaimed water.