The Effects of Cycling Levels of 17β-Estradiol and Progesterone on the Magnitude of Temporomandibular Joint-Induced Nociception
The Effects of Cycling Levels of 17β-Estradiol and Progesterone on the Magnitude of Temporomandibular Joint-Induced Nociception
复制标题
DOI：
10.1210/en.2008-1707
复制
发表时间：
2009-08-01
期刊：
影响因子：
4.8
通讯作者：
Bellinger, L. L.
中科院分区：
文献类型：
--
作者：
Kramer, P. R.;Bellinger, L. L.
据报道，女性颞下颌关节（TMJ）疼痛的发生率更高，这表明性腺激素可能在这种情况下起作用。然而，17 -雌二醇（E2）和孕酮（P4）在TMJ疼痛中的确切作用尚不完全清楚。我们通过两个实验来确定E2和P4在发情周期不同阶段TMJ痛觉中的不同作用。卵巢切除（OVX）大鼠以生理浓度E2或P4循环。E2循环大鼠在发情2日（低E2状态）或发情前（高E2状态）早晨接受双侧颞下颌关节注射生理盐水（SAL）或完全弗氏佐剂（CFA）。作为对照，OVX大鼠（没有卵巢E2和替代）注射SAL或CFA。颞下颌关节痛觉的测量采用了一种经过验证的新方法，其中进食时间的增加与颞下颌关节深层痛觉的强度直接相关。E2实验中，在OVX组中，CFA注射增加了TMJ痛觉，而SAL不增加，但在发情-2期效果不明显，在发情前更不明显。在P4实验中，大鼠在发情期-2期（P4小峰期结束）注射TMJ CFA后，未表现出TMJ伤害感受的增加，而在发情期（P4大峰期）、发情期（P4低峰期）和孕中期（P4低峰期）注射的大鼠TMJ伤害感受的增加相似。激素浓度不影响TMJ IL-1 β、IL-6、C-C基序配体20、C-X-C基序配体2或三叉神经节降钙素基因相关肽。E2在发情前期的高生理浓度和P4在发情期的低生理浓度减弱或消除了cfa诱导的TMJ痛觉。结果提示，E2和P4浓度可影响cfa诱导的大鼠颞下颌关节痛觉。（内分泌学150:3680-3689,2009）
A greater incidence of temporomandibular joint (TMJ) pain is reported in females, suggesting that gonadal hormones may play a role in this condition. However, the exact roles of 17 beta-estradiol (E2) and progesterone (P4) in TMJ pain are not completely known. Two experiments were performed to determine the separate roles of E2 and P4 in TMJ nociception at various stages of the estrous cycle. Ovariectomized (OVX) rats were cycled with physiological concentrations of E2 or P4. The E2-cycled rats then received bilateral TMJ injections of saline (SAL) or complete Freund's adjuvant (CFA) on the morning of diestrus-2 (low E2 condition) or proestrus (high E2 condition). As a control, OVX rats (no ovarian E2 and no replacement) were injected with SAL or CFA. The TMJ nociception was measured using a validated novel method in which an increase in meal duration directly correlated to the intensity of deep TMJ nociception. In the E2 experiment, CFA injection, but not SAL, increased TMJ nociception in the OVX group, but the effect was less pronounced in diestrus-2 and even less in proestrus. In the P4 experiment, the rats receiving TMJ CFA in diestrus-2 (end of minor P4 surge) did not show an increase in TMJ nociception, whereas the rats injected in proestrus (major P4 surge), estrus (low P4), and metestrus (low P4) had similar increases in TMJ nociception. The hormones' concentration did not affect TMJ IL-1 beta, IL-6, C-C motif ligand 20, or C-X-C motif ligand 2 or the trigeminal ganglia calcitonin gene-related peptide. The high physiological concentrations of E2 observed at proestrus and the low P4 concentrations observed at diestrus-2 attenuated or eliminated CFA-induced TMJ nociception. The results suggest that the cyclic estrous cycle concentrations of E2 and P4 can influence CFA-induced TMJ nociception in the rat. (Endocrinology 150: 3680-3689, 2009)