Stem Cells of the Apical Papilla Regulate Trigeminal Neurite Outgrowth and Targeting through a BDNF-Dependent Mechanism
Stem Cells of the Apical Papilla Regulate Trigeminal Neurite Outgrowth and Targeting through a BDNF-Dependent Mechanism
复制标题
DOI：
10.1089/ten.tea.2013.0347
复制
发表时间：
2014-12-01
影响因子：
4.1
通讯作者：
Diogenes, Anibal
中科院分区：
文献类型：
--
作者：
de Almeida, Jose Flavio A.;Chen, Paul;Diogenes, Anibal
再生牙髓手术已成为治疗牙髓坏死的未成熟牙齿的一种有价值的选择。除了解决根周病变和促进持续的根发育外，在一些再生临床病例中还观察到积极的活力测试。重要的是，再生组织对电刺激的积极反应需要将根周轴突靶向到先前空的根管空间中。然而，这一过程发生的机制在很大程度上是未知的。由于根尖乳头干细胞（SCAP）已被提出填充根管再生牙髓手术后，我们假设，SCAP调节神经突生长和轴突靶向。为了验证这一假设，我们建立了人SCAP和大鼠三叉神经元的原代共培养物，并使用ELISA和共聚焦显微镜进行神经突生长测定，以确定增加浓度的SCAP对总神经突生长和轴突靶向的影响。此外，我们使用基质胶皮下植入试验评估了SCAP是否在体内诱发轴突靶向。通过ANOVA和Bonferroni事后检验分析数据，并将显著性设定为p
Regenerative endodontic procedures have become a valuable alternative for the treatment of immature teeth with pulp necrosis. In addition to resolution of periradicular pathosis and promotion of continued root development, positive vitality testing has been observed in some regenerative clinical cases. Importantly, the positive response to electric stimulation of the regenerated tissue requires targeting of periradicular axons into the previously empty root canal space. However, the mechanism by which this process occurs is largely unknown. Since stem cells of the apical papilla (SCAP) have been proposed to populate the root canal following regenerative endodontic procedures, we hypothesized that SCAP regulate neurite outgrowth and axonal targeting. To test this hypothesis, we established primary co-cultures of human SCAP and rat trigeminal neurons, and performed neurite outgrowth assays using ELISA and confocal microscopy to determine the effect of increasing concentration of SCAP on the total neurite outgrowth and axonal targeting. In addition, we evaluated whether SCAP evoked axonal targeting in vivo using a matrigel subcutaneous implant assay. Data were analyzed by ANOVA with Bonferroni's post hoc test, and significance was set at p