Oral cancer in vivo gene expression profiling assisted by laser capture microdissection and microarray analysis
Oral cancer in vivo gene expression profiling assisted by laser capture microdissection and microarray analysis
复制标题
DOI：
10.1038/sj.onc.1204685
复制
发表时间：
2001-09-27
期刊：
影响因子：
8
通讯作者：
Wong, DTW
中科院分区：
文献类型：
--
作者：
Alevizos, I;Mahadevappa, M;Wong, DTW
对激光捕获的肿瘤和正常口腔上皮细胞进行了大规模的基因表达谱分析，并在高密度寡核苷酸芯片上进行了分析。大约有600个基因被发现与口腔癌相关。这些口腔癌相关基因包括癌基因、肿瘤抑制因子、转录因子、外源酶、转移蛋白、分化标记物以及与口腔癌无关的基因。创建的数据库提供了口腔癌发生过程中可验证的基因表达的全球概况，揭示了已知基因以及先前未涉及口腔癌的基因的潜在作用。
Large scale gene expression profiling was carried out on laser capture microdissected (LCM) tumor and normal oral epithelial cells and analysed on high-density oligonucleotide microarrays. About 600 genes were found to be oral cancer associated. These oral cancer associated genes include oncogenes, tumor suppressors, transcription factors, xenobiotic enzymes, metastatic proteins, differentiation markers, and genes that have not been implicated in oral cancer. The database created provides a verifiable global profile of gene expression during oral carcinogenesis, revealing the potential role of known genes as well as genes that have not been previously implicated in oral cancer.