pH-sensitive, cationic liposomes: A new synthetic virus-like vector
pH-sensitive, cationic liposomes: A new synthetic virus-like vector
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DOI：
10.1038/nbt0696-760
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发表时间：
1996-06-01
影响因子：
46.9
通讯作者：
Wolff, JA
中科院分区：
文献类型：
--
作者：
Budker, V;Gurevich, V;Wolff, JA
我们描述了使用阳离子、pH 敏感脂质体来介导 DNA 有效转移到培养物中的各种细胞中，合成了含有 pK 在 4.5 至 8 生理范围内的胺的阳离子脂质，并将其与二油酰磷脂酰乙醇胺掺入脂质体中。酸性条件促进了这些阳离子、pH 敏感脂质体的 DNA 结合、DNA 掺入和 DNA 诱导融合。培养细胞中的转染效率取决于内体酸化，其方式类似于酸诱导内体释放病毒。这些脂质体构成了一种有前途的新型基因治疗试剂。
We describe the use of cationic, pH-sensitive liposomes to mediate the efficient transfer of DNA into a variety of cells in culture, Cationic lipids, containing an amine with a pK within the physiologic range of 4.5 to 8, were synthesized and incorporated with dioleoylphosphatidylethanolamine into liposomes, Acid conditions promoted DNA-binding, DNA-incorporation, and DNA-induced fusion by these cationic, pH-sensitive liposomes. Transfection efficiency in cultured cells was dependent on endosomal acidification in a manner akin to acidic-induced endosomal release of viruses, These liposomes constitute a promising new class of reagents for gene therapy.