Immunoglobulins are the major glycoproteins involved in the modifications of total serum N-glycome in cirrhotic patients
Immunoglobulins are the major glycoproteins involved in the modifications of total serum N-glycome in cirrhotic patients
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DOI：
10.1002/prca.200900133
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发表时间：
2010-04-01
影响因子：
2
通讯作者：
Morelle, Willy
中科院分区：
文献类型：
--
作者：
Klein, Andre;Carre, Yoann;Morelle, Willy
目的：人血清糖蛋白的N-糖基化修饰已在肝硬变中被描述。为了确定携带这些修饰的糖蛋白，并确定它们在肝硬变患者血清总N-糖蛋白(TSNG)修饰中的影响，我们对肝硬变患者的免疫球蛋白、转铁蛋白、1抗胰蛋白酶和结合珠蛋白进行了糖基化分析。实验设计：采用基于MS、2-DE和亲和层析的策略，对14例肝硬变患者和11名健康对照的免疫球蛋白G、转铁蛋白、1抗胰蛋白酶和结合珠蛋白进行糖基化分析。结果：我们证实了肝细胞和血浆细胞分泌的糖蛋白的N-糖基化发生了改变，TSNG的主要修饰是由免疫球蛋白A和G携带的。结论和临床意义：寻找血糖生物标志物作为肝活检的替代方法来评估慢性肝病的纤维化是极其重要的。免疫球蛋白糖基化的变化是影响这些分子的TSNG和Fc效应性质的主要修饰，当然也有助于纤维化的病理生理。
Purpose: N-glycosylation modifications in human serum glycoproteins have been described in hepatic cirrhosis. To identify the glycoproteins carrying these modifications and to determine their influences in the modification of the total serum N-glycome (TSNG) in cirrhotic patients, we have performed the glycosylation analysis of immunoglobulins, transferrin, 1 antitrypsin and haptoglobin of patients who have developed cirrhosis.Experimental design: The glycosylation analysis of immunoglobulins G, transferrin, 1 antitrypsin and haptoglobin of 14 patients who have developed cirrhosis and 11 healthy controls was performed using strategies based on MS, 2-DE and affinity chromatography.Results: We demonstrated that the N-glycosylation of both hepatic and plasma cell secreted glycoproteins is modified, and that the major modifications of TSNG are carried by immunoglobulins A and G.Conclusions and clinical Relevance: The search for glycomic biomarkers used as an alternative to liver biopsy for the assessment of fibrosis in chronic liver disease is extremely important. Variations in the glycosylation of immunoglobulins are responsible for the main modifications affecting the TSNG and effector properties of the Fc of these molecules, and certainly contribute to the pathophysiology of fibrosis.