Intracellular localization of group 3 LEA proteins in embryos of Artemia franciscana
Intracellular localization of group 3 LEA proteins in embryos of Artemia franciscana
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DOI：
10.1016/j.tice.2014.09.004
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发表时间：
2014-12-01
期刊：
影响因子：
2.6
通讯作者：
Hand, Steven C.
中科院分区：
文献类型：
--
作者：
Boswell, Leaf C.;Hand, Steven C.
脱水生物体在干旱条件下积累了胚胎发育晚期丰富蛋白（莱亚），提高了水分胁迫下的存活率。在这项研究中，我们为AfrLEA 2和AfrLEA 3m（及其亚型）在卤虫脱水胚胎中的亚细胞定位提供了第一个直接证据。免疫组织化学显示AfrLEA2驻留在细胞质和细胞核中，并且四种AfrLEA3m蛋白定位于细胞核。线粒体、细胞核和细胞质组分的蛋白质印迹支持细胞位置。莱亚蛋白存在于A. franciscana胚胎表明，为了使生物体在干燥胁迫下存活，需要保护细胞的许多区域中的生物结构，并且可以部分地解释为什么单个生物体表达多种不同的莱亚蛋白。(C)2014爱思唯尔有限公司版权所有。
Late embryogenesis abundant (LEA) proteins are accumulated by anhydrobiotic organisms in response to desiccation and improve survivorship during water stress. In this study we provide the first direct evidence for the subcellular localizations of AfrLEA2 and AfrLEA3m (and its subforms) in anhydrobiotic embryos of Artemia franciscana. Immunohistochemistry shows AfrLEA2 to reside in the cytoplasm and nucleus, and the four AfrLEA3m proteins to be localized to the mitochondrion. Cellular locations are supported by Western blots of mitochondrial, nuclear and cytoplasmic fractions. The presence of LEA proteins in multiple subcellular compartments of A. franciscana embryos suggests the need to protect biological structures in many areas of a cell in order for an organism to survive desiccation stress, and may explain in part why a multitude of different LEA proteins are expressed by a single organism. (C) 2014 Elsevier Ltd. All rights reserved.