Exploration of human miRNA target genes in neuronal differentiation.
Exploration of human miRNA target genes in neuronal differentiation.
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DOI：
10.1093/nass/49.1.341
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发表时间：
2005-09
期刊：
影响因子：
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通讯作者：
Y. Fukuda;H. Kawasaki;K. Taira
中科院分区：
文献类型：
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作者：
Y. Fukuda;H. Kawasaki;K. Taira
microRNA（miRNAs）是以序列特异性方式抑制蛋白质翻译的内源性非编码RNA分子。我们对TPA刺激后的神经母细胞瘤细胞SH-SY 5 Y中的180种人前体miRNA进行了微阵列分析。12个pre-miRNAs被TPA刺激上调。我们还探索了与神经元分化相关的miRNA靶基因。一些miRNA与Notch 1基因的3 'UTR互补，Notch 1基因是神经元分化的调节因子，荧光素酶测定表明，这些miRNA的过表达降低了含有Notch 1 - 3' UTR序列的报告基因的荧光素酶活性。我们的研究结果表明，miRNA可能与TPA诱导的信号通路和Notch 1基因的表达相关。
MicroRNAs (miRNAs) are endogenous non-coding RNA molecules that inhibit protein translation in a sequence specific manner. We carried out microarray analyses of 180 human pre-miRNAs in neuroblastoma cell, SH-SY5Y, following stimulation by TPA. Twelve of the pre-miRNAs were up-regulated by the TPA stimulation. We also explored miRNA target genes associated with neuronal differentiation. Some miRNAs have complementarity with 3'UTR of the Notch1 gene, a regulator of neuronal differentiation, and luciferase assay showed that overexpression of these miRNAs reduced the luciferase activity of reporter genes containing the Notch1-3'UTR sequence. Our results suggest that miRNAs can be associated with TPA induced signalling pathways and expression of Notch1 gene.