HIGH CELL-DENSITY CULTIVATION OF ESCHERICHIA-COLI-W USING SUCROSE AS A CARBON SOURCE
HIGH CELL-DENSITY CULTIVATION OF ESCHERICHIA-COLI-W USING SUCROSE AS A CARBON SOURCE
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DOI：
10.1007/bf00131766
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发表时间：
1993-09-01
影响因子：
2.7
通讯作者：
CHANG, HN
中科院分区：
文献类型：
--
作者：
LEE, SY;CHANG, HN
大肠杆菌W在以蔗糖为唯一碳源的化学成分确定的培养基中以pH-stat补料分批模式培养。当pH升高时，在一定时间内补加20 g蔗糖+0.57 g MgSO_4·7 H_2 O，36 h内细胞终浓度可达105 g/L。采用同样的策略，携带pSYL 104的重组菌株也可以在48小时内生长至125 g/L。
Escherichia coli W was cultivated in the pH-stat fed-batch mode in a chemically defined medium containing sucrose as a sole carbon source. A final cell concentration of 105 g/L could be obtained in 36 hrs by feeding 20 g sucrose+0.57 g MgSO47H2O for a definite on-time upon the pH rise. Using the same strategy, a recombinant strain harboring pSYL104 could also be grown to 125 g/L in 48 hrs.