Role of protein conformational dynamics in the catalysis by 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase.
Role of protein conformational dynamics in the catalysis by 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase.
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DOI：
10.2174/092986611794654003
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发表时间：
2011-04
影响因子：
1.6
通讯作者：
Ji X
中科院分区：
文献类型：
--
作者：
Yan H;Ji X
Enzymatic catalysis has conflicting structural requirements of the enzyme. In order for the enzyme to form a Michaelis complex, the enzyme must be in an open conformation so that the substrate can get into its active center. On the other hand, in order to maximize the stabilization of the transition state of the enzymatic reaction, the enzyme must be in a closed conformation to maximize its interactions with the transition state. The conflicting structural requirements can be resolved by a flexible active center that can sample both open and closed conformational states. For a bisubstrate enzyme, the Michaelis complex consists of two substrates in addition to the enzyme. The enzyme must remain flexible upon the binding of the first substrate so that the second substrate can get into the active center. The active center is fully assembled and stabilized only when both substrates bind to the enzyme. However, the side-chain positions of the catalytic residues in the Michaelis complex are still not optimally aligned for the stabilization of the transition state, which lasts only approximately 10−13 s. The instantaneous and optimal alignment of catalytic groups for the transition state stabilization requires a dynamic enzyme, not an enzyme which undergoes a large scale of movements but an enzyme which permits at least a small scale of adjustment of catalytic group positions. This review will summarize the structure, catalytic mechanism, and dynamic properties of 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase and examine the role of protein conformational dynamics in the catalysis of a bisubstrate enzymatic reaction.
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影响因子：
3.4
作者：
Yang, R;Lee, MC;Duan, Y
通讯作者：
Duan, Y
影响因子：
2.9
作者：
Lescop, Ewen;Lu, Zhenwei;Jin, Changwen
通讯作者：
Jin, Changwen
影响因子：
2.9
作者：
Li, Guangyu;Felczak, Krzysztof;Yan, Honggao
通讯作者：
Yan, Honggao
DOI：
10.1002/9780470123195.ch6
发表时间：
1999-01-01
期刊：
ADVANCES IN ENZYMOLOGY, VOL 73
影响因子：
--
作者：
Mildvan, AS;Weber, DJ;Abeygunawardana, C
通讯作者：
Abeygunawardana, C
影响因子：
4.8
作者：
Bermingham, A;Bottomley, JR;Derrick, JP
通讯作者：
Derrick, JP