Ultrastructural description of taurine-like immunoreactive cells and processes in the rat hippocampus.
Ultrastructural description of taurine-like immunoreactive cells and processes in the rat hippocampus.
复制标题
大鼠海马牛磺酸样免疫反应细胞和过程的超微结构描述。
DOI：
10.1002/syn.890040108
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Beitz,AJ
中科院分区：
文献类型：
--
作者：
Clements,JR;Magnusson,KR;Beitz,AJ
使用与 KLH 缀合的抗牛磺酸单克隆抗体来识别和描述大鼠海马中的牛磺酸样免疫反应过程。对灌注大鼠的组织进行处理，用于牛磺酸的免疫组织化学可视化，并包埋用于电子显微镜检查。对来自齿状回、CA3 和 CA1 三个区域的代表性组织样本进行切片、检查和拍照。在齿状回中，颗粒细胞和锥体篮细胞均具有牛磺酸样免疫反应性。齿状回分子层中的一些轴突末端以及门中的一些苔藓纤维束也具有牛磺酸样免疫反应性。在 CA3 区域，锥体神经元和神经胶质细胞均呈牛磺酸样免疫反应。放射层中的一些小直径轴突末端和透明层中的一些苔藓纤维束具有牛磺酸样免疫反应性。在 CA1 中，锥体神经元和一些神经胶质细胞具有强烈的牛磺酸样免疫反应性。在放射层和定向层中发现了一些免疫反应性轴突末端。在所有区域中，树突染色占主导地位。我们的结果支持这样的假设：虽然牛磺酸可能在海马末梢的一小部分中充当神经递质，但其主要功能可能是作为神经调节剂或离子调节剂。
A monoclonal antibody against taurine conjugated to KLH was used to identify and describe taurine‐like immunoreactive processes in the rat hippocampus. Tissue from perfused rats was processed for immunohistochemical visualization of taurine and embedded for electron microscopy. Representative tissue samples from three regions, the dentate gyrus, CA3, and CA1, were sectioned, examined, and photographed. In the dentate gyrus, both granule cells and pyramidal basket cells were taurine‐like immunoreactive. Some axon terminals in the dentate gyrus molecular layer as well as some mossy fiber boutons in the hilus were also taurine‐like immunoreactive. In the CA3 region both pyramidal neurons and glial cells were taurine‐like immunoreactive. A few small‐diameter axon terminals in stratum radiatum and some mossy fiber boutons in stratum lucidum were taurine‐like immunoreactive. In CA1, pyramidal neurons and some glia were intensely taurine‐like immunoreactive. A few immunoreactive axon terminals were seen in stratum radiatum and stratum oriens. In all regions, dendritic staining predominated. Our results support the hypothesis that while taurine may act as a neurotransmitter in a small portion of hippocampal terminals, its main function is probably as a neuromodulator or ionic regulator.