Submergence-responsive MicroRNAs are potentially involved in the regulation of morphological and metabolic adaptations in maize root cells.
Submergence-responsive MicroRNAs are potentially involved in the regulation of morphological and metabolic adaptations in maize root cells.
复制标题
DOI：
10.1093/aob/mcn129
复制
发表时间：
2008-10
期刊：
影响因子：
4.2
通讯作者：
Zuxin Zhang;Liya Wei;Xiling Zou;Y. Tao;Zhijie Liu;Yong-lian Zheng
中科院分区：
文献类型：
--
作者：
Zuxin Zhang;Liya Wei;Xiling Zou;Y. Tao;Zhijie Liu;Yong-lian Zheng
背景和目的当玉米植株被淹没或遭受土壤淹水时，就会出现厌氧或低氧条件。玉米在低氧条件下的生存在很大程度上依赖于代谢、生理和形态适应策略，其调控机制尚不清楚。MicroRNAs(MiRNAs)在转录后水平上在响应不利的生物或非生物胁迫中发挥关键作用。本研究的目的是了解淹水响应miRNAs及其在淹水玉米根中的潜在作用。方法使用含有植物miRNA的定制muParaflo微流控阵列(miRbase：http://microrna.sanger.ac.uk)探针)寻找差异表达的miRNA。从处理过的根中提取的小RNA与微阵列杂交。用RT-PCR对小RNA的靶点及其顺式作用元件进行预测和分析。关键结果基因芯片数据显示，来自9个玉米和其他一些植物miRNA家族的39个miRNAs的表达水平发生了显著变化(P<；0.01)。在不同的淹水时间点上鉴定了四种表达谱。早期诱导了ZMA-miRNA166、ZMA-miRNA167、ZMA-miRNA171和OSA-miRNA396-like，并预测它们的靶基因编码重要的转录因子，包括HD-ZIP、生长素反应因子、SCL和WRKY结构域蛋白。ZMA-miR159、ath-miR395、PTC-miR474和OSA-MIR528-like在淹水初期被抑制，并在淹水24 h后被诱导。这些miRNAs的预测靶标涉及碳水化合物和能量代谢，包括淀粉合成酶、转化酶、苹果酸酶和ATPase。此外，许多预测的目标涉及消除活性氧物种和乙醛。总体而言，诱导的miRNAs的大多数靶标都含有顺式作用元件，这是厌氧反应或激素诱导所必需的。结论淹水响应miRNAs在转录后水平上参与了玉米根系代谢、生理和形态适应的调节。
BACKGROUND AND AIMS Anaerobic or low oxygen conditions occur when maize plants are submerged or subjected to flooding of the soil. Maize survival under low oxygen conditions is largely dependent on metabolic, physiological and morphological adaptation strategies; the regulation mechanisms of which remain unknown. MicroRNAs (miRNAs) play critical roles in the response to adverse biotic or abiotic stresses at the post-transcriptional level. The aim of this study was to understand submergence-responsive miRNAs and their potential roles in submerged maize roots. METHODS A custom muParaflo microfluidic array containing plant miRNA (miRBase: http://microrna.sanger.ac.uk) probes was used to explore differentially expressed miRNAs. Small RNAs from treated roots were hybridized with the microarray. The targets and their cis-acting elements of small RNA were predicted and analysed by RT-PCR. KEY RESULTS Microarray data revealed that the expression levels of 39 miRNAs from nine maize and some other plant miRNA families were significantly altered (P < 0.01). Four expression profiles were identified across different submergence time-points. The zma-miRNA166, zma-miRNA167, zma-miRNA171 and osa-miRNA396-like were induced in the early phase, and their target genes were predicted to encode important transcription factors, including; HD-ZIP, auxin response factor, SCL and the WRKY domain protein. zma-miR159, ath-miR395-like, ptc-miR474-like and osa-miR528-like were reduced at the early submergence phase and induced after 24 h of submergence. The predicted targets for these miRNAs were involved in carbohydrate and energy metabolism, including starch synthase, invertase, malic enzyme and ATPase. In addition, many of the predicted targets were involved in the elimination of reactive oxygen species and acetaldehyde. Overall, most of the targets of induced miRNAs contained the cis-acting element, which is essential for the anaerobic response or hormone induction. CONCLUSIONS Submergence-responsive miRNAs are involved in the regulation of metabolic, physiological and morphological adaptations of maize roots at the post-transcriptional level.