A minimally invasive optical trapping system to understand cellular interactions at onset of an immune response.
A minimally invasive optical trapping system to understand cellular interactions at onset of an immune response.
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DOI：
10.1371/journal.pone.0188581
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Wright AJ
中科院分区：
文献类型：
--
作者：
Glass DG;McAlinden N;Millington OR;Wright AJ
t细胞和抗原提呈细胞是适应性免疫反应系统的重要组成部分，它们如何相互作用对于身体如何有效地对抗感染或对疫苗作出反应至关重要。许多研究细胞间相互作用力的实验工作都着眼于大量细胞样本的平均特性，或者应用显微镜对这些细胞之间的动态接触进行成像。在本文中，我们提出了一种新的光捕获技术，用于询问这种相互作用的力并测量单细胞水平上的相对相互作用力。三点光学陷阱用于直接操纵感兴趣的细胞，而不会将诸如珠子之类的异物引入系统。光阱用于直接控制细胞-细胞接触的开始，并随后在定义的时间点终止相互作用。分离免疫细胞对所需的激光束功率是确定的，并与光阱施加的力有关。作为概念的证明，树突状细胞和特定t细胞之间的相互作用力的抗原特异性增加被证明。此外，研究表明，当t细胞信号传导被阻断时，这种相互作用力完全消失。因此，使用光学捕获在单细胞水平上询问细胞相互作用而无需引入诸如珠子之类的异物的潜力得到了清楚的证明。
T-cells and antigen presenting cells are an essential part of the adaptive immune response system and how they interact is crucial in how the body effectively fights infection or responds to vaccines. Much of the experimental work studying interaction forces between cells has looked at the average properties of bulk samples of cells or applied microscopy to image the dynamic contact between these cells. In this paper we present a novel optical trapping technique for interrogating the force of this interaction and measuring relative interaction forces at the single-cell level. A triple-spot optical trap is used to directly manipulate the cells of interest without introducing foreign bodies such as beads to the system. The optical trap is used to directly control the initiation of cell-cell contact and, subsequently to terminate the interaction at a defined time point. The laser beam power required to separate immune cell pairs is determined and correlates with the force applied by the optical trap. As proof of concept, the antigen-specific increase in interaction force between a dendritic cell and a specific T-cell is demonstrated. Furthermore, it is demonstrated that this interaction force is completely abrogated when T-cell signalling is blocked. As a result the potential of using optical trapping to interrogate cellular interactions at the single cell level without the need to introduce foreign bodies such as beads is clearly demonstrated.
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影响因子：
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DOI：
10.1073/pnas.2628040100
发表时间：
2003-03-04
影响因子：
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作者：
Miller, MJ;Wei, SH;Parker, I
通讯作者：
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