Characterization and Optimization of Injectable In Situ Crosslinked Chitosan-Genipin Hydrogels.
Characterization and Optimization of Injectable In Situ Crosslinked Chitosan-Genipin Hydrogels.
复制标题
可注射原位交联壳聚糖-京尼平水凝胶的表征和优化。
DOI：
10.1002/mabi.202200505
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发表时间：
2023
影响因子：
4.6
通讯作者：
Caldorera-Moore,MaryE
中科院分区：
文献类型：
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作者：
Priddy-Arrington,TylerR;Edwards,ReaganE;Colley,ClaireE;Nguyen,MarissaM;Hamilton-Adaire,Tess;Caldorera-Moore,MaryE
近年来，可注射原位交联水凝胶因其微创应用和适应环境的能力而受到越来越多的关注。目前的原位交联壳聚糖水凝胶要么机械强度大，生物相容性差，由于有毒的交联剂生物降解有限，要么由于交联度不足，水凝胶机械性能较弱，生物降解过快。在此，作者开发并表征了一种热驱动、可注射的壳聚糖-京尼平水凝胶，该水凝胶能够在37℃下进行原位交联，具有机械强度高、可生物降解并保持高度生物相容性的特点。天然交联剂京尼平被用作热驱动的无毒交联剂。研究了壳聚糖-京尼平水凝胶的交联动力学、可注射性、粘弹性、溶胀和pH响应以及与人角质形成细胞的生物相容性。所开发的壳聚糖-京尼平水凝胶在37℃下成功交联，表现出温度敏感性。水凝胶在生物相关的环境中降解之前，在几周内保持高比例的膨胀，在保持生物可降解性的同时显示出机械稳定性。长期的细胞活性研究表明，壳聚糖-京尼平水凝胶在7天内具有良好的生物相容性，包括在水凝胶交联期。总体而言，这些发现支持开发一种可注射的、原位交联的壳聚糖-京尼平水凝胶，用于微创生物医学应用。
In recent years, there has been an increased interest in injectable, in situ crosslinking hydrogels due to their minimally invasive application and ability to conform to their environment. Current in situ crosslinking chitosan hydrogels are either mechanically robust with poor biocompatibility and limited biodegradation due to toxic crosslinking agents or the hydrogels are mechanically weak and undergo biodegradation too rapidly due to insufficient crosslinking. Herein, the authors developed and characterized a thermally‐driven, injectable chitosan‐genipin hydrogel capable of in situ crosslinking at 37 °C that is mechanically robust, biodegradable, and maintain high biocompatibility. The natural crosslinker genipin is utilized as a thermally‐driven, non‐toxic crosslinking agent. The chitosan‐genipin hydrogel's crosslinking kinetics, injectability, viscoelasticity, swelling and pH response, and biocompatibility against human keratinocyte cells are characterized. The developed chitosan‐genipin hydrogels are successfully crosslinked at 37 °C, demonstrating temperature sensitivity. The hydrogels maintained a high percentage of swelling over several weeks before degrading in biologically relevant environments, demonstrating mechanical stability while remaining biodegradable. Long‐term cell viability studies demonstrated that chitosan‐genipin hydrogels have excellent biocompatibility over 7 days, including during the hydrogel crosslinking phase. Overall, these findings support the development of an injectable, in situ crosslinking chitosan‐genipin hydrogel for minimally invasive biomedical applications.