Relationship between metabolic enzyme activities and bioaccumulation kinetics of PAHs in zebrafish (Danio rerio).
Relationship between metabolic enzyme activities and bioaccumulation kinetics of PAHs in zebrafish (Danio rerio).
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DOI：
10.1016/j.jes.2017.03.037
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发表时间：
2017-04
影响因子：
6.9
通讯作者：
Haotian Wang;Yayuan Li;X. Xia;Xinyue Xiong
中科院分区：
文献类型：
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作者：
Haotian Wang;Yayuan Li;X. Xia;Xinyue Xiong
多环芳烃（PAHs）在水生生物体内的积累和代谢已被广泛研究。然而，缺乏研究同时调查这两个过程中，这两个过程之间的相互作用是了解到目前为止。研究了多环芳烃在斑马鱼体内的生物富集动力学和代谢酶活性，包括总细胞色素P450和总超氧化物歧化酶。采用被动给药系统，将菲和蒽的混合物在恒定浓度下暴露于成熟斑马鱼16 d。结果表明，暴露1.5 d后，斑马鱼体内多环芳烃浓度达到峰值，随后逐渐下降。接触12天后达到生物累积平衡。吸收速率常数（ku）和消除速率常数（ke）均在峰值后呈下降趋势。斑马鱼体内多环芳烃浓度与代谢酶活性的变化存在相互作用的关系。CYP和T-SOD活性随PAH浓度的增加先升高后降低，当PAH浓度达到峰值时，活性降至最低。当多环芳烃达到生物积累平衡时，CYP和T-SOD活性也达到稳定状态。总的来说，暴露于多环芳烃后，CYP和T-SOD活性被激活。斑马鱼体内PAH浓度在峰值后下降可能是由于ku的大幅下降和CYP活性的变化。本研究表明，多环芳烃在水生生物体内的生物累积动力学与代谢酶活性之间存在着相互作用的关系。
Many studies have investigated bioaccumulation and metabolism of polycyclic aromatic hydrocarbons (PAHs) in aquatic organisms. However, lack of studies investigated both processes simultaneously, and the interaction between these two processes is less understood so far. This study investigated the bioaccumulation kinetics of PAHs and metabolic enzyme activities, including total cytochrome P450 (CYPs) and total superoxide dismutase (T-SOD), in zebrafish. Mature zebrafish were exposed to the mixture of phenanthrene and anthracene under constant concentration maintained by passive dosing systems for 16 days. The results showed that PAH concentrations in zebrafish experienced a peak value after exposure for 1.5 days, and then decreased gradually. The bioaccumulation equilibrium was achieved after exposure for 12 days. Both of the uptake rate constants (ku) and the elimination rate constants (ke) decreased after the peak value. The variation of PAH concentrations and metabolic enzyme activities in zebrafish had an interactive relationship. The activities of CYPs and T-SOD increased initially with the increase of PAH concentrations, but decreased to the lowest state when PAH concentrations reached the peak value. When the bioaccumulation equilibrium of PAHs was achieved, CYPs and T-SOD activities also reached the steady state. In general, CYPs and T-SOD activities were activated after exposure to PAHs. The decrease of PAH concentrations in zebrafish after the peak value may be attributed to the great drop ofkuand the variation of CYPs activities. This study suggests that an interactive relationship exists between bioaccumulation kinetics of PAHs and metabolic enzyme activities in aquatic organisms.