Evaluation of long term stability of seeded bacteria in a bio-enhanced activated carbon filter used for treating drinking water
Evaluation of long term stability of seeded bacteria in a bio-enhanced activated carbon filter used for treating drinking water
复制标题
DOI：
10.1016/j.ibiod.2013.03.019
复制
发表时间：
2013-11
影响因子：
4.8
通讯作者：
Duoying Zhang;Weiguang Li;H. Gong;Lei Zhang;X. Gong;Baiyin Liu
中科院分区：
文献类型：
--
作者：
Duoying Zhang;Weiguang Li;H. Gong;Lei Zhang;X. Gong;Baiyin Liu
采用固定化5种细菌的生物强化活性炭（BEAC）滤池，考察了接种细菌的稳定性。每天连续分析过滤器的TOC和铵去除效率。采用扫描电镜、ATP试验和DGGE技术分别对生物膜、生物活性和细菌群落结构进行了评价。在初始阶段（0天），低丰度的细菌导致低的生物活性（202.97 ng ATP g− 1碳），这导致低TOC和铵去除率（63.98%和30.68%）。第120天时，随着生物活性的增加，TOC和氨氮的去除率也随之提高（增加到1436.8 ng ATP g-1碳），分别达到81.47%和51.97%。DGGE结果显示，从BEAC运行的第0-120天，5个接种的细菌是稳定的，变化不大。第180天时，DGGE图谱中出现了4株土著菌，影响了TOC的去除效果和生物活性。荧光原位杂交结果表明，在第180天时，接种菌占细菌总数的92.98%，在BEAC滤池中占优势。在这项研究中，BEAC与相对稳定的接种细菌是一种有前途的方法，以保持过滤器的性能。
A bio-enhanced activated carbon (BEAC) filter immobilized with 5 bacteria was operated for 180 days to investigate the seeded bacterial stability. The TOC and ammonium removal efficiency of the filter was analyzed continuously every day. The biofilm, bioactivity and bacterial community structure was evaluated by SEM, ATP test and DGGE respectively. At the initial stage (0 day), low abundance of the bacteria led to low bioactivity (202.97 ng ATP g−1carbon), which resulted in low TOC and ammonium removal efficiency (63.98% and 30.68%). On the 120th day, The TOC and ammonium removal efficiency improved with the increase of bioactivity (increased to 1436.8 ng ATP g−1carbon) and climbed to 81.47% and 51.97%, respectively. DGGE results showed that from day 0–120 of the BEAC operation, 5 seeded bacteria were stable with little change. On the 180th day, there were 4 indigenous bacteria appeared in DGGE profiles, which affected TOC removal efficiency and bioactivity. Fluorescence in situ hybridization results showed that, on the 180th day, the seeded bacteria were 92.98% of total count of bacteria, which were dominant in BEAC filter. In this study, BEAC with relatively stable seeded bacteria is a promising approach to maintain performance of the filter.