Expression of ubiquitin-conjugating enzyme E2C/UbcH10 in astrocytic tumors
Expression of ubiquitin-conjugating enzyme E2C/UbcH10 in astrocytic tumors
复制标题
泛素结合酶 E2C/UbcH10 在星形细胞肿瘤中的表达
DOI：
10.1016/j.brainres.2008.01.037
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发表时间：
2008-03-27
期刊：
影响因子：
2.9
通讯作者：
Han, Huan-Xing
中科院分区：
文献类型：
--
作者：
Jiang, Lei;Huang, Cheng-Guang;Han, Huan-Xing
UbcH10 是通过有丝分裂纺锤体组装检查点途径进行细胞周期进程的关键调节因子之一。最近，在多种恶性肿瘤中已证实 UbcH10 异常高表达。然而，其在星形细胞癌发生中的作用尚不明确。本研究探讨了UbcH10基因的剪接模式及其在不同级别星形细胞瘤中的表达状况。因此，通过 RT-PCR 和序列分析，在星形细胞瘤和正常脑组织中检测到 UbcH10 剪接变体 I（GenBank 登录号 NM_007019）。通过定量实时 PCR 分析确定，高级别星形细胞瘤与低级别星形细胞瘤（分别为 64.33 +/- 60.98 与 8.36 +/- 8.15；p=0.000）或正常对照（分别为 64.33 +/- 60.98 与 1.00 +/- 137；p=0.000）中 UbcH10 mRNA 的表达水平升高。同样，免疫组织化学研究显示，与低级别肿瘤（分别为 10.53 +/- 5.79% 与 4.23 +/- 2.85%；p = 0.000）或正常对照（分别为 10.53 +/- 5.79% 与 0.0 +/- 0.0%；p=0.000）相比，高级星形细胞瘤中的 UbcH10 标记指数增加，并且，还注意到 UbcH10 免疫反应性和 Ki-67 免疫染色（Spearman r=0.63，p
UbcH10 is one of the key regulators of cell cycle progression through the mitotic spindle assembly checkpoint pathway. Recently, aberrantly high UbcH10 expression has been demonstrated in a variety of malignancies. However, its role in astrocytic carcinogenesis is not well defined. This study investigated the splice pattern of the UbcH10 gene and its expression status in astrocytomas of different grades. Consequently, UbcH10 splice variant I (GenBank accession nos. NM_007019) was detected in astrocytomas and normal brain tissues by RT-PCR and sequence analysis. Expression levels of UbcH10 mRNA were elevated in high-versus low-grade astrocytomas (64.33 +/- 60.98 vs 8.36 +/- 8.15, respectively; p=0.000) or normal controls (64.33 +/- 60.98 vs 1.00 +/- 137, respectively; p=0.000), as determined by quantitative real time PCR analysis. Similarly, immunohistochemistry study showed increased UbcH10 labelling index in high-grade astrocytomas versus low-grade tumors (10.53 +/- 5.79% vs 4.23 +/- 2.85%, respectively; p = 0.000) or normal controls (10.53 +/- 5.79% vs 0.0 +/- 0.0%, respectively; p=0.000) and, a positive correlation between UbcH10 immunoreactivity and Ki-67 immunostaining was also noted (Spearman r=0.63, p