Direct Assessments of the Antioxidant Effects of the Novel Collagen Peptide on Reactive Oxygen Species Using Electron Spin Resonance Spectroscopy
Direct Assessments of the Antioxidant Effects of the Novel Collagen Peptide on Reactive Oxygen Species Using Electron Spin Resonance Spectroscopy
复制标题
DOI：
10.1254/jphs.10321fp
复制
发表时间：
2011-05-01
影响因子：
3.5
通讯作者：
Lee, Masaichi-Chang-Il
中科院分区：
文献类型：
--
作者：
Kobayashi, Kyo;Maehata, Yojiro;Lee, Masaichi-Chang-Il
在本研究中，我们评估了胃蛋白酶处理的新型胶原肽（P-NCP）对活性氧（ROS）如羟基自由基（HO中心点）、超氧阴离子自由基（O-2（中心点-））和单线态氧（O-1（2））的抗氧化作用，以及对紫外线（UV）照射后的人成纤维细胞活力的影响。我们用电子自旋共振证实，P-NCP直接抑制HO中心点和O-1（2）。此外，向成纤维细胞中添加P-NCP以剂量依赖性方式抑制由UVA（400 - 315 nm）照射诱导的细胞死亡。此外，富含Gly、Pro、Hyp、Glu、Ala和Arg的肽组分对O-1（2）具有抗氧化作用。我们发现Gly、Hyp、Glu和Ala直接清除O-1（2）。这些结果表明，包括Gly，Hyp，Glu和Ala的肽序列可能在P-NCP对O-1的抗氧化作用中起关键作用（2）。结果表明，P-NCP具有抗氧化作用，可抑制与ROS有关的光老化。
In the present study, we evaluated the antioxidant effects of a pepsin-treated novel collagen peptide (P-NCP) on reactive oxygen species (ROS) such as hydroxyl radical (HO center dot), superoxide anion radical (O-2(center dot-)), and singlet oxygen (O-1(2)), and the effects on cell viability after ultraviolet ray (UV) irradiation of human fibroblasts. We confirmed, using electron spin resonance, that P-NCP directly inhibited HO center dot and O-1(2). Furthermore, addition of P-NCP to fibroblasts inhibited cell death induced by UVA (400 - 315 nm) irradiation in a dose-dependent manner. In addition, the antioxidant effect on O-1(2) was observed in the peptide fractions rich in Gly, Pro, Hyp, Glu, Ala, and Arg. We found that Gly, Hyp, Glu, and Ala directly scavenged O-1(2). These results indicated that a peptide sequence including Gly, Hyp, Glu, and Ala could play a key role in the antioxidant effects of P-NCP on O-1(2). It was suggested that P-NCP can inhibit photo-aging related to ROS owing to its antioxidant effects.