Effect of surface chemistry of novel templates on crystallization of proteins
Effect of surface chemistry of novel templates on crystallization of proteins
复制标题
DOI：
10.1016/j.ces.2012.01.049
复制
发表时间：
2012-07-30
影响因子：
4.7
通讯作者：
Heng, Jerry Y. Y.
中科院分区：
文献类型：
--
作者：
Tsekova, Daniela S.;Williams, Daryl R.;Heng, Jerry Y. Y.
本文报道了溶菌酶、苏氨酸、过氧化氢酶和铁蛋白四种模型蛋白质在新型有机模板上的非均相结晶。介绍了玻璃基板的化学表面改性。通过接触角的测量表征了所形成的有机层，并测定了它们的色散和极性表面能分量。使用悬滴技术完成结晶研究。发现基质表面化学影响蛋白质结晶的几个方面：形成的晶体的数量、晶体的尺寸和晶体形态。所获得的数据证实了理论上的建议，即具有特定表面化学的模板可以成功地用于控制蛋白质晶体的成核和形态。(C)2012爱思唯尔有限公司保留所有权利。
The heterogeneous crystallization of four model proteins (Lysozyme, Thaumatine, Catalase and Ferritin) on novel organic templates is reported. The chemical surface modification of glass substrates is described. The organic layers formed are characterized by contact angles' measurement with their dispersive and polar surface energy components determinated. Crystallization studies were completed using the hanging drop technique. The substrate surface chemistry was found to influence several aspects of protein crystallization: the number of crystals formed, the sizes of crystals and the crystal morphology. Data obtained confirm the theoretical suggestions that templates with specific surface chemistry can be successfully used for the control of nucleation and morphology of protein crystals. (C) 2012 Elsevier Ltd. All rights reserved.