A novel marker for terminal Schwann cells, homocysteine-responsive ER-resident protein, as isolated by a single cell PCR-differential display
A novel marker for terminal Schwann cells, homocysteine-responsive ER-resident protein, as isolated by a single cell PCR-differential display
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DOI：
10.1016/s0006-291x(03)01499-2
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发表时间：
2003-09-05
影响因子：
3.1
通讯作者：
Fushiki, S
中科院分区：
文献类型：
--
作者：
Oda, R;Yaoi, T;Fushiki, S
覆盖运动神经元末梢的末端施温细胞（TSCs）在维持神经肌肉连接以及神经损伤后的修复过程中发挥重要作用。然而，由于TSCs数量少，分析它们的分子特征仍然未知。我们建立了一种选择性、高效地收集tsc的方法，从而可以正确地进行cDNA分析。我们比较了髓鞘Schwarm细胞（MSCs）和TSCs中1-2%的全mrna的表达，结果表明，大约三分之一的条带可以归类为细胞类型特异性条带。因此，从基因表达的角度来看，TSCs构成了一个独特的实体。由于同源半胱氨酸反应性ER-resident protein （Herp）是属于tsc特异性带的cDNA克隆之一，原位杂交证实Herp mRNA在运动神经末梢的tsc中表达，而在发育和成年大鼠的MSCs中不表达。总之，我们已经能够识别Herp作为一种新的TSCs分子标记物。(C) 2003 Elsevier Inc.版权所有。
Terminal Schwarm cells (TSCs) that cover motor neuron terminals are known to play important roles in maintaining neuromuscular junctions, as well as in the repair process after nerve injury. However, molecular characteristics of TSCs remain unknown, because of the difficulties in analyzing them due to their paucity. We have established a method of selectively and efficiently collecting TSCs so that cDNA analysis can be done properly. The expression of 1-2% of whole mRNAs was compared between myelinating Schwarm cells (MSCs) and TSCs, and it turned out that approximately one-third of the bands could be categorized as cell-type-specific bands. TSCs thus constitute a distinct entity from the viewpoint of gene expression. As one of the cDNA clones belonging to TSC-specific bands was identified homocysteine-responsive ER-resident protein (Herp), and in situ hybridization confirmed that Herp mRNA is expressed in TSCs on motor nerve terminals but not in MSCs, both in developing and adult rats. In conclusion, we have been able to identify Herp as a novel molecular marker for TSCs. (C) 2003 Elsevier Inc. All rights reserved.