SINDBIS VIRUS-INDUCED CYTOPATHIC EFFECT IN CLONES OF AEDES-ALBOPICTUS (SINGH) CELLS
SINDBIS VIRUS-INDUCED CYTOPATHIC EFFECT IN CLONES OF AEDES-ALBOPICTUS (SINGH) CELLS
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DOI：
10.1016/s0042-6822(77)80014-7
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发表时间：
1977-01-01
期刊：
影响因子：
3.7
通讯作者：
STOLLAR, V
中科院分区：
文献类型：
--
作者：
SARVER, N;STOLLAR, V
分离出白纹伊蚊细胞的克隆，与衍生它们的未克隆亲本细胞群不同，该克隆在感染辛德毕斯病毒（SV）后表现出广泛的细胞病变效应（CPE）。其中一种克隆，白纹伊蚊 LT C-7，在感染东部马脑炎病毒和水泡性口炎病毒后表现出 CPE。来自克隆 LT C-7 和克隆 AIS C-3（一种 CPE 抗性克隆）的辛德毕斯病毒产量没有显着差异。 LT C-7 细胞中的病毒 RNA 合成始终高于 AIS C-3 细胞。在 LT C-7 细胞中，病毒诱导的 CPE 和病毒 RNA 合成在 34°C 时均得到增强。 C 相对于 28°C C;在AIS C-3细胞中，28°C时没有CPE。 C 或 34 度。 C和病毒RNA合成在较高温度下并未增强。病毒感染导致AIS C-3细胞中宿主RNA合成受到轻微抑制，但LT C-7细胞中宿主RNA合成受到显着抑制。
Clones of A. albopictus cells were isolated which, unlike the uncloned parental cell populations from which they were derived, show extensive cytopathic effect (CPE) after infection with Sindbis virus (SV). One such clone, A. albopictus LT C-7, manifested CPE upon infection with eastern equine encephalitis virus and vesicular stomatitis virus. Yields of Sindbis virus from clone LT C-7 and from clone AIS C-3, a CPE-resistant clone, were not significantly different. Viral RNA synthesis was consistently greater in the LT C-7 cells than in the AIS C-3 cells. In the LT C-7 cells both virus-induced CPE and viral RNA synthesis were enhanced at 34.degree. C relative to 28.degree. C; in the AIS C-3 cells there was no CPE at 28.degree. C or 34.degree. C and viral RNA synthesis was not enhanced at the higher temperature. Viral infection led to a mild inhibition of host RNA synthesis in the AIS C-3 cells but to a marked inhibition in the LT C-7 cells.