Identifying biochemical phenotypic differences between cryptic species.
Identifying biochemical phenotypic differences between cryptic species.
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识别隐秘物种之间的生化表型差异。
DOI：
10.1098/rsbl.2014.0615
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发表时间：
2014
期刊：
影响因子：
3.3
通讯作者：
Liebeke M
中科院分区：
文献类型：
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作者：
Liebeke M
分子遗传学方法可以区分以前似乎是单一物种的不同进化谱系，但并不总是清楚这些神秘物种之间存在哪些功能差异。我们使用代谢组学的方法来配置文件的生化表型（代谢型）之间的差异，两个假定的神秘物种的蚯蚓红蚯蚓。没有直接的谱系代谢物生物标志物，即在一个谱系中没有始终处于较高浓度的代谢物。然而，多变量方法鉴定了少量的代谢物，这些代谢物一起有助于区分谱系，包括不常见的代谢物，如N ε-三甲基赖氨酸，其通常在高浓度下不存在。这种方法可能是有用的特征功能性状差异，特别是因为它适用于基本上任何物种组，无论其基因组测序状态。
Molecular genetic methods can distinguish divergent evolutionary lineages in what previously appeared to be single species, but it is not always clear what functional differences exist between such cryptic species. We used a metabolomic approach to profile biochemical phenotype (metabotype) differences between two putative cryptic species of the earthwormLumbricus rubellus. There were no straightforward metabolite biomarkers of lineage, i.e. no metabolites that were always at higher concentration in one lineage. Multivariate methods, however, identified a small number of metabolites that together helped distinguish the lineages, including uncommon metabolites such asNε-trimethyllysine, which is not usually found at high concentrations. This approach could be useful for characterizing functional trait differences, especially as it is applicable to essentially any species group, irrespective of its genome sequencing status.