Longitudinal Changes in Retinal Blood Flow in a Feline Retinal Vein Occlusion Model as Measured by Doppler Optical Coherence Tomography and Optical Coherence Tomography Angiography
Longitudinal Changes in Retinal Blood Flow in a Feline Retinal Vein Occlusion Model as Measured by Doppler Optical Coherence Tomography and Optical Coherence Tomography Angiography
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DOI：
10.1167/iovs.61.2.34
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发表时间：
2020-02-01
影响因子：
4.4
通讯作者：
Yoshida, Akitoshi
中科院分区：
文献类型：
--
作者：
Wada, Takanari;Song, Youngseok;Yoshida, Akitoshi
目的.采用多普勒光学相干断层扫描（DOCT）和光学相干断层血管成像（OCTA）技术，观察猫视网膜血流阻塞（RVO）模型中视网膜血流（RBF）的纵向变化和毛细血管结构的变化。用氩绿色激光光凝（PC）诱导6只猫6只眼RVO。通过DOCT流量计在一级视网膜动脉和静脉处测量RBF，并通过OCTA在之前（基线时）、PC后即刻以及之后第1、4、7和14天评估闭塞血管（12 x 12和3 x 3 mm）周围毛细血管的结构变化。观察期间监测全身和眼部参数。结果。PC前后任何全身或眼部参数均无显著差异。动脉RBF在第1天显著增加（160.6 +/- 8.6% vs.基线，P < 0.001），并在第1天至第14天后降至基线以下。静脉RBF在PC后立即降低（17.4 +/- 9.6% vs基线，P = 0.001），然后逐渐升高，但未恢复至基线。OCTA显示PC后即刻至第1天视网膜小静脉扩张。侧支血管在第4天开始形成，第7天成熟，第14天被修剪，形成的侧支血管与正常视网膜小静脉直径一样成熟。在诱导RVO后1天内，随着动脉RBF的增加，微静脉逐渐扩张形成侧支血管，表明侧支血管来源于现有的毛细血管网络，而不是新血管形成。
PURPOSE. We aimed to observe longitudinal changes in retinal blood flow (RBF) and structural transformations in capillaries using Doppler optical coherence tomography (DOCT) and optical coherence tomography angiography (OCTA) in a feline retinal blood occlusion (RVO) model.METHODS. RVO was induced by argon green laser photocoagulation (PC) in six eyes of six cats. RBF was measured at a first-order retinal artery and vein by a DOCT flowmeter, and structural changes in the capillaries around the occluded vessels (12 x 12 and 3 x 3 mm) were assessed by OCTA before (at baseline); immediately after PC; and on days 1, 4, 7, and 14 thereafter. Systemic and ocular parameters were monitored during the observation period.RESULTS. There were no significant differences in any systemic or ocular parameters before and after PC. Arterial RBF increased significantly on day 1 (160.6 +/- 8.6% vs. baseline, P < 0.001) and decreased below baseline after day 1 through 14. Venous RBF decreased immediately after PC (17.4 +/- 9.6% vs. baseline, P = 0.001) and then gradually increased afterwards, but did not return to baseline. OCTA showed dilatation of retinal venules immediately after PC to day 1. Collateral vessels began to form on day 4, had matured by day 7, and were pruned on day 14, which formed as mature as normal retinal venule diameters.CONCLUSIONS. With increasing arterial RBF within 1 day after inducing RVO, venules gradually expanded to form collateral vessels, suggesting that collateral vessels originate from existing capillary networks, not neovascularization.