Serotype, Genotype, and Antimicrobial Susceptibility Profiles of Salmonella from Chicken Farms in Shanghai
Serotype, Genotype, and Antimicrobial Susceptibility Profiles of Salmonella from Chicken Farms in Shanghai
复制标题
上海养鸡场沙门氏菌血清型、基因型及药敏特征
DOI：
10.4315/0362-028x-73.3.562
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发表时间：
2010-03-01
影响因子：
2
通讯作者：
Shi, Xian-Ming
中科院分区：
文献类型：
--
作者：
Liu, Wei-Bing;Chen, Jing;Shi, Xian-Ming
2005年3月至2006年10月期间，从上海5个养鸡场采集了550份样品。共550份样品，其中25份样品检出沙门氏菌阳性，其中500份来自健康鸡粪便，50份来自病鸡粪便。 API 20E 鉴定试剂盒对 25 种假定沙门氏菌分离株进行了确认。通过抗血清凝集试验对这些分离株进行血清分型，显示出七种血清型；通过多位点序列分型对这些分离株进行基因分型，显示出六种序列类型 (ST) 模式（即 ST-11、ST-19、ST-92、ST-96、ST-290 和 ST-367）。多位点序列分型数据显示，其中一些从不同农场分离的菌株可能具有相同的 ST，并且可能来自同一来源。确定了这些菌株对 14 种抗菌药物的敏感性；大多数分离株（25 株中的 13 株）对多西环素和四环素耐药，两个分离株对头孢噻肟和头孢他啶耐药，但没有一个对庆大霉素或卡那霉素耐药。
Five hundred fifty samples were collected from five chicken farms in Shanghai during March 2005 to October 2006. Twenty-five samples tested positive for Salmonella from a total of 550 samples, of which 500 were obtained from feces of healthy chickens and 50 were obtained from diseased chicks. The 25 presumptive Salmonella isolates were confirmed by the API 20E identification kit. Serotyping of these isolates by agglutination tests with antiserum displayed seven serovars; genotyping of these isolates with multilocus sequence typing demonstrated six sequence type (ST) patterns (i.e., ST-11, ST-19, ST-92, ST-96, ST-290, and ST-367). The multilocus sequence typing data revealed that some of these strains, isolated from different farms, might have the same ST and might come from the same source. The susceptibilities of these strains to 14 antimicrobials were determined; most of the isolates (13 of 25) were resistant to doxycycline and tetracycline, and two isolates were resistant to cefotaxime and ceftazidime, but none was resistant to gentamicin or kanamycin.