Optimizing CRISPR/Cas9-based gene manipulation in echinoderms.
Optimizing CRISPR/Cas9-based gene manipulation in echinoderms.
复制标题
优化棘皮动物中基于 CRISPR/Cas9 的基因操作。
DOI：
10.1016/j.ydbio.2022.07.008
复制
发表时间：
2022
影响因子：
2.7
通讯作者：
Wessel, GM
中科院分区：
文献类型：
--
作者：
Oulhen N;Pieplow C;Perillo M;Gregory P;Wessel, GM
新技术的影响可以通过它的广泛使用来衡量。以前只依赖药理学方法或使用吗啉代反义寡核苷酸（MASO）技术的研究人员现在能够更有效地应用CRISPR-Cas9来研究他们选择的模型生物中的生物学问题。向基于CRISPR的新方法的过渡可以首先通过标准化协议和应用教育来加强。在这里，我们总结了我们在海胆和海星星星中优化CRISPR-Cas9技术的结果，并就如何在棘皮动物中建立CRISPR-Cas9实验和解释结果提供了建议。我们的目标是通过这些方案和分享其他实验室的成功案例，降低激活障碍，以便更多的实验室可以在这些重要的动物中应用CRISPR-Cas9技术。
The impact of new technology can be appreciated by how broadly it is used. Investigators that previously relied only on pharmacological approaches or the use of morpholino antisense oligonucleotide (MASO) technologies are now able to apply CRISPR-Cas9 to study biological problems in their model organism of choice much more effectively. The transitions to new CRISPR-based approaches could be enhanced, first, by standardized protocols and education in their applications. Here we summarize our results for optimizing the CRISPR-Cas9 technology in a sea urchin and a sea star, and provide advice on how to set up CRISPR-Cas9 experiments and interpret the results in echinoderms. Our goal through these protocols and sharing examples of success by other labs is to lower the activation barrier so that more laboratories can apply CRISPR-Cas9 technologies in these important animals.