Metaproteomics reveal that rapid perturbations in organic matter prioritize functional restructuring over taxonomy in western Arctic Ocean microbiomes
Metaproteomics reveal that rapid perturbations in organic matter prioritize functional restructuring over taxonomy in western Arctic Ocean microbiomes
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DOI：
10.1038/s41396-019-0503-z
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发表时间：
2020-01-01
期刊：
影响因子：
11
通讯作者：
Nunn, Brook L.
中科院分区：
文献类型：
--
作者：
Mikan, Molly P.;Harvey, H. Rodger;Nunn, Brook L.
我们在10天的船上孵化期间检查了两个北极微生物群的代谢蛋白质组图谱，以直接跟踪对模拟藻华和寡营养对照的早期功能和分类反应。使用一种新的基于多肽的富集分析，观察到与碳和氮循环相关的生物和分子功能的显著变化(p值和lt；0.01)。在两种有机质条件下的第一天内，白令海峡表面微生物群增加了蛋白质合成、碳水化合物降解和细胞氧化还原过程，而降低了C1代谢。分类学研究表明，在选定的类群利用和代谢细胞内的氮之前，核心微生物群通过吸收碳来对藻类底物做出集体反应。楚科奇海底水微生物群的孵化表现出相似的，但延迟了对相同处理的功能反应。尽管24个功能术语在实验处理之间共享，但其余响应的时间和程度变化很大，表明有机质扰动在微生物类群水平上的分类分布改变之前指导了群落功能。这两个海洋微生物群落的动态响应对于北冰洋对有机扰动的响应的时间和大小以及群落一级的功能如何预测海洋的生物地球化学梯度具有重要的影响。
We examined metaproteome profiles from two Arctic microbiomes during 10-day shipboard incubations to directly track early functional and taxonomic responses to a simulated algal bloom and an oligotrophic control. Using a novel peptide-based enrichment analysis, significant changes (p-value < 0.01) in biological and molecular functions associated with carbon and nitrogen recycling were observed. Within the first day under both organic matter conditions, Bering Strait surface microbiomes increased protein synthesis, carbohydrate degradation, and cellular redox processes while decreasing C1 metabolism. Taxonomic assignments revealed that the core microbiome collectively responded to algal substrates by assimilating carbon before select taxa utilize and metabolize nitrogen intracellularly. Incubations of Chukchi Sea bottom water microbiomes showed similar, but delayed functional responses to identical treatments. Although 24 functional terms were shared between experimental treatments, the timing, and degree of the remaining responses were highly variable, showing that organic matter perturbation directs community functionality prior to alterations to the taxonomic distribution at the microbiome class level. The dynamic responses of these two oceanic microbial communities have important implications for timing and magnitude of responses to organic perturbations within the Arctic Ocean and how community-level functions may forecast biogeochemical gradients in oceans.