Smartphone-Based Fluorescent Sensing Platforms for Point-of-Care Ocular Lactoferrin Detection
Smartphone-Based Fluorescent Sensing Platforms for Point-of-Care Ocular Lactoferrin Detection
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DOI：
10.1016/j.snb.2022.133128
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发表时间：
2022-12
期刊：
影响因子：
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通讯作者：
Yuqi Shi;Yihan Zhang;Yubing Hu;Rosalia Moreddu;Zichen Fan;N. Jiang;A. Yetisen
中科院分区：
文献类型：
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作者：
Yuqi Shi;Yihan Zhang;Yubing Hu;Rosalia Moreddu;Zichen Fan;N. Jiang;A. Yetisen
乳铁蛋白是一种重要的糖蛋白，占泪液蛋白组成的主要成分。泪液乳铁蛋白已被指示为眼部健康筛查（例如干眼病诊断）中的潜在生物标志物。荧光生物传感器是目前诊断方法的理想替代品，因为它们具有高选择性和灵敏度，以及在护理点（POC）平台上的快速和具有成本效益的检测技术。在此，检测荧光乳铁蛋白传感并将其应用于硝酸纤维素膜、毛细管和接触透镜平台上，与开发的智能手机软件合作用于数据收集和分析。基于三价铽（TbCl3）的荧光传感器被集成到不同的平台中，包括硝酸纤维素膜、毛细管和隐形眼镜，并在人工泪液中进行测试。采用与智能手机相机集成的定制3D打印读出设备进行图像采集和读出。乳铁蛋白传感器的检测范围可以在0至5 mg mL− 1之间变化，具有强线性关系，并且对于侧向流传感产生0.57 mg mL− 1的检测限（LOD），对于毛细管传感产生0.12 mg mL− 1的检测限，对于接触透镜传感产生0.44 mg mL− 1的检测限。接触透镜传感器获得最高线性回归，而毛细管传感器获得最低LOD。这项工作可以为在POC进行乳铁蛋白监测铺平道路，并可能导致在英国国家卫生服务（NHS）住院，以进行未来的临床试验和检查。
Lactoferrin is a critical glycoprotein that accounts for the major component in tear protein composition. Tear lactoferrin has been indicated as a potential biomarker in ocular health screening, such as dry eye disease diagnosis. Fluorescent biosensors are a desirable alternative to current diagnostic methods, due to their high selectivity and sensitivity, and rapid and cost-effective detection technologies at point-of-care (POC) platforms. Herein, fluorescent lactoferrin sensing is examined and applied on nitrocellulose membrane, capillary tube, and contact lens platforms in cooperation with a developed smartphone software for data collection and analysis. Fluorescent sensors based on trivalent terbium (TbCl3) are integrated into different platforms, including nitrocellulose membranes, capillary tubes, and contact lenses, and tested in artificial tear fluid. A bespoke 3D printed readout device integrated with a smartphone camera was employed for image acquisition and readout. The detection range of the lactoferrin sensors could be varied from 0 to 5 mg mL−1with a strong linear relation and yielded a limit of detection (LOD) of 0.57 mg mL−1for lateral flow sensing, 0.12 mg mL−1for capillary tube sensing, and 0.44 mg mL−1for contact lens sensing. The contact lens sensor obtained the highest linear regression, while the capillary tube sensor achieved a lowest LOD. This work could pave the way towards accessible lactoferrin monitoring at POC and could induce hospitalization at the UK national health service (NHS) for future clinical trials and examinations.