Ductular hepatocytes. Evidence for a bile ductular cell origin in furan-treated rats.
Ductular hepatocytes. Evidence for a bile ductular cell origin in furan-treated rats.
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发表时间：
1994-08
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通讯作者：
A. Sirica;T. W. Gainey;V. Mumaw
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作者：
A. Sirica;T. W. Gainey;V. Mumaw
幼年Fischer 344大鼠接受呋喃严重肝毒性治疗后，在萎缩的右肝内出现广泛的胆管反应，胆管样结构由不同成熟阶段的胆管上皮细胞和典型的单个胆管肝细胞组成。与正常胆管相比，这些胆管样结构完全被基底膜包围，基底膜对层粘连蛋白和IV型胶原都有很强的免疫组织化学染色反应。所有胆管上皮细胞和胆管肝细胞的γ-谷氨酰转肽酶和细胞角蛋白8免疫组织化学染色均呈强阳性。细胞角蛋白19在所有胆管上皮细胞中也呈强阳性表达，但仅在部分胆管肝细胞中呈强阳性表达，而后者仅白蛋白免疫组织化学染色阳性，胞浆内含有过氧化物体。单个胆管状肝细胞与相邻的胆管上皮细胞之间形成细胞连接。在这一新的严重肝损伤模型中，导管肝细胞的细胞和细胞核直径范围及其表型特征与罕见的胆管样细胞向更成熟的导管肝细胞过渡的分化是一致的。
Cholangiolar-like structures composed of biliary epithelial cells and typically a single ductular hepatocytic cell in various stages of maturation appeared in association with an extensive bile ductular reaction within the atrophied right liver lobe of young adult Fischer 344 rats that had been subjected to a severely hepatotoxic treatment with furan. In contrast to normal cholangioles, these cholangiolar-like structures were completely surrounded by a basement membrane, which gave strong immunohistochemical staining reactions for both laminin and type IV collagen. All of the biliary epithelial cells and ductular hepatocytic cells exhibited a strongly positive immunohistochemical staining for gamma-glutamyl transpeptidase and for cytokeratin 8. Cytokeratin 19 was also strongly expressed in all of the biliary epithelial cells, but in just some of the ductular hepatocytic cells, whereas only the latter cells were immunohistochemically positive for albumin and contained peroxisomes in their cytoplasm. Cell junctions were formed between individual ductular hepatocytic cells and adjacent biliary epithelial cells of the same cholangiolar-like structures. In this novel model of severe hepatic injury, the range of cell and nuclear diameters characterizing the ductular hepatocytic cells, together with their phenotypic features, are consistent with a differentiation of rare bile ductular-like cells to transitional ductular cells to more mature ductular hepatocytes.